1mgv

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[[Image:1mgv.gif|left|200px]]
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{{STRUCTURE_1mgv| PDB=1mgv | SCENE= }}
{{STRUCTURE_1mgv| PDB=1mgv | SCENE= }}
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'''Crystal Structure of the R391A Mutant of 7,8-Diaminopelargonic Acid Synthase'''
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===Crystal Structure of the R391A Mutant of 7,8-Diaminopelargonic Acid Synthase===
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==Overview==
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7,8-diaminopelargonic acid (DAPA) synthase (EC 2.6.1.62) is a pyridoxal phosphate (PLP)-dependent transaminase that catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form DAPA in the antepenultimate step in the biosynthesis of biotin. The wild-type enzyme has a steady-state kcat value of 0.013 s(-1), and the K(m) values for SAM and KAPA are 150 and &lt;2 microM, respectively. The k(max) and apparent K(m) values for the half-reaction of the PLP form of the enzyme with SAM are 0.016 s(-1) and 300 microM, respectively, while those for the reaction with DAPA are 0.79 s(-1) and 1 microM. The R391A mutant enzyme exhibits near wild-type kinetic parameters in the reaction with SAM, while the apparent K(m) for DAPA is increased 180-fold. The 2.1 A crystal structure of the R391A mutant enzyme shows that the mutation does not significantly alter the structure. These results indicate that the conserved arginine residue is not required for binding the alpha-amino acid SAM, but it is important for recognition of DAPA.
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(as it appears on PubMed at http://www.pubmed.gov), where 12379100 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12379100}}
==About this Structure==
==About this Structure==
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[[Category: Homodimer]]
[[Category: Homodimer]]
[[Category: Subclass ii]]
[[Category: Subclass ii]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:00:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:55:59 2008''

Revision as of 20:56, 2 July 2008

Image:1mgv.png

Template:STRUCTURE 1mgv

Crystal Structure of the R391A Mutant of 7,8-Diaminopelargonic Acid Synthase

Template:ABSTRACT PUBMED 12379100

About this Structure

1MGV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The dual-specific active site of 7,8-diaminopelargonic acid synthase and the effect of the R391A mutation., Eliot AC, Sandmark J, Schneider G, Kirsch JF, Biochemistry. 2002 Oct 22;41(42):12582-9. PMID:12379100

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