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1mhn

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{{STRUCTURE_1mhn| PDB=1mhn | SCENE= }}
{{STRUCTURE_1mhn| PDB=1mhn | SCENE= }}
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'''High resolution crystal structure of the SMN Tudor domain'''
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===High resolution crystal structure of the SMN Tudor domain===
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==Overview==
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The SMN protein, which is linked to spinal muscular atrophy (SMA), plays an important role in the assembly of the spliceosomal small nuclear ribonucleoprotein complexes. This function requires binding of SMN to the arginine-glycine (RG) rich C-terminal tails of the Sm proteins, which contain symmetrically dimethylated arginine residues (sDMA) in vivo. Using NMR titrations, we show that the SMN Tudor domain recognizes these sDMAs in the methylated RG repeats. Upon complex formation a cluster of conserved aromatic residues in the SMN Tudor domain interacts with the sDMA methyl groups. We present two high resolution structures of the uncomplexed SMN Tudor domain, a 1.8A crystal structure and an NMR structure that has been refined against a large number of backbone and side-chain residual dipolar couplings. The backbone conformation of both structures is very similar, however, differences are observed for the cluster of conserved aromatic side-chains in the sDMA binding pocket. In order to validate these variations we introduce a novel application of residual dipolar couplings for aromatic rings. We show that structural information can be derived from aromatic ring residual dipolar couplings, even in the presence of internal motions such as ring flipping. These residual dipolar couplings and ring current shifts independently confirm that the SMN Tudor domain adopts two different conformations in the sDMA binding pocket. The observed structural variations may play a role for the recognition of sDMAs.
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{{ABSTRACT_PUBMED_12628254}}
==About this Structure==
==About this Structure==
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[[Category: Smn]]
[[Category: Smn]]
[[Category: Spinal muscular atrophy]]
[[Category: Spinal muscular atrophy]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:02:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:59:05 2008''

Revision as of 20:59, 2 July 2008

Image:1mhn.png

Template:STRUCTURE 1mhn

High resolution crystal structure of the SMN Tudor domain

Template:ABSTRACT PUBMED 12628254

About this Structure

1MHN is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

High-resolution X-ray and NMR structures of the SMN Tudor domain: conformational variation in the binding site for symmetrically dimethylated arginine residues., Sprangers R, Groves MR, Sinning I, Sattler M, J Mol Biol. 2003 Mar 21;327(2):507-20. PMID:12628254

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