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1mi8

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[[Image:1mi8.gif|left|200px]]
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{{STRUCTURE_1mi8| PDB=1mi8 | SCENE= }}
{{STRUCTURE_1mi8| PDB=1mi8 | SCENE= }}
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'''2.0 Angstrom crystal structure of a DnaB intein from Synechocystis sp. PCC 6803'''
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===2.0 Angstrom crystal structure of a DnaB intein from Synechocystis sp. PCC 6803===
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==Overview==
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We have determined the crystal structure of a 154-residue intein derived from the dnaB gene of Synechocystis sp. strain PCC6803 and refined it to a 2.0-A resolution. The x-ray structure suggests that this intein possesses two catalytic sites that appear to be separately responsible for splicing and cleavage of the N- and C-terminal scissile bonds. The conserved intein block F residues are the important components of a catalytic site for side chain cyclization of the last intein residue, Asn-154. The data suggest that the imidazole ring of His-143 is involved in the activation of the side chain Ndelta atom of Asn-154, leading to a nucleophilic attack on the carbonyl carbon of Asn-154. Substitution of His-143 with Ala or Gln resulted in the inhibition of C-terminal cleavage. His-153, Asp-136, and a water molecule appear to constitute an oxyanion binding site by contacting the carbonyl oxygen of Asn-154 to stabilize the transition state. The structure and mutagenesis data also support that the close contact between the hydroxyl groups of Thr-138 and Ser-155, whose side chain participates in an S --&gt; O acyl shift, plays an important role in the nucleophile orientation. Our structural modeling suggests that this catalytic module is conserved in the C-terminal subdomains of inteins from diverse organisms.
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(as it appears on PubMed at http://www.pubmed.gov), where 12878593 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12878593}}
==About this Structure==
==About this Structure==
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[[Category: Xu, M.]]
[[Category: Xu, M.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:05:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:01:48 2008''

Revision as of 21:01, 2 July 2008

Image:1mi8.png

Template:STRUCTURE 1mi8

2.0 Angstrom crystal structure of a DnaB intein from Synechocystis sp. PCC 6803

Template:ABSTRACT PUBMED 12878593

About this Structure

1MI8 is a Single protein structure of sequence from Synechocystis sp.. Full crystallographic information is available from OCA.

Reference

Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing., Ding Y, Xu MQ, Ghosh I, Chen X, Ferrandon S, Lesage G, Rao Z, J Biol Chem. 2003 Oct 3;278(40):39133-42. Epub 2003 Jul 23. PMID:12878593

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