1mk8

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{{STRUCTURE_1mk8| PDB=1mk8 | SCENE= }}
{{STRUCTURE_1mk8| PDB=1mk8 | SCENE= }}
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'''Crystal Structure of a Mutant Cytochrome c Peroxidase showing a Novel Trp-Tyr Covalent Cross-link'''
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===Crystal Structure of a Mutant Cytochrome c Peroxidase showing a Novel Trp-Tyr Covalent Cross-link===
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==Overview==
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The crystal structure of a cytochrome c peroxidase mutant where the distal catalytic His52 is converted to Tyr reveals that the tyrosine side-chain forms a covalent bond with the indole ring nitrogen atom of Trp51. We hypothesize that this novel bond results from peroxide activation by the heme iron followed by oxidation of Trp51 and Tyr52. This hypothesis has been tested by incorporation of a redox-inactive Zn-protoporphyrin into the protein, and the resulting crystal structure shows the absence of a Trp51-Tyr52 cross-link. Instead, the Tyr52 side-chain orients away from the heme active-site pocket, which requires a substantial rearrangement of residues 72-80 and 134-144. Additional experiments where heme-containing crystals of the mutant were treated with peroxide support our hypothesis that this novel Trp-Tyr cross-link is a peroxide-dependent process mediated by the heme iron.
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(as it appears on PubMed at http://www.pubmed.gov), where 12684005 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12684005}}
==About this Structure==
==About this Structure==
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[[Category: Trp cation radical]]
[[Category: Trp cation radical]]
[[Category: Tryptophan-tyrosine cross-link]]
[[Category: Tryptophan-tyrosine cross-link]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:16:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:10:11 2008''

Revision as of 21:10, 2 July 2008

Image:1mk8.png

Template:STRUCTURE 1mk8

Crystal Structure of a Mutant Cytochrome c Peroxidase showing a Novel Trp-Tyr Covalent Cross-link

Template:ABSTRACT PUBMED 12684005

About this Structure

1MK8 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

A novel heme and peroxide-dependent tryptophan-tyrosine cross-link in a mutant of cytochrome c peroxidase., Bhaskar B, Immoos CE, Shimizu H, Sulc F, Farmer PJ, Poulos TL, J Mol Biol. 2003 Apr 18;328(1):157-66. PMID:12684005

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