1mp1
From Proteopedia
(New page: 200px<br /> <applet load="1mp1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mp1" /> '''Solution structure of the PWI motif from SR...) |
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'''Solution structure of the PWI motif from SRm160'''<br /> | '''Solution structure of the PWI motif from SRm160'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The PWI motif is a highly conserved domain of unknown function in the | + | The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids. Deletion of the motif prevents SRm160 from binding RNA and stimulating 3'-end cleavage, and its substitution with a heterologous RNA-binding domain restores these functions. The NMR solution structure of the SRm160-PWI motif reveals a novel, four-helix bundle and represents the first example of an alpha-helical fold that can bind single-stranded (ss)RNA. Structure-guided mutagenesis indicates that the same surface is involved in RNA and DNA binding and requires the cooperative action of a highly conserved, adjacent basic region. Thus, the PWI motif is a novel type of nucleic acid-binding domain that likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3'-end formation. |
==About this Structure== | ==About this Structure== | ||
| - | 1MP1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1MP1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arrowsmith, C | + | [[Category: Arrowsmith, C H.]] |
| - | [[Category: Blencowe, B | + | [[Category: Blencowe, B J.]] |
[[Category: Bowman, J.]] | [[Category: Bowman, J.]] | ||
[[Category: Cox, B.]] | [[Category: Cox, B.]] | ||
| - | [[Category: Graveley, B | + | [[Category: Graveley, B R.]] |
[[Category: Lambermon, M.]] | [[Category: Lambermon, M.]] | ||
[[Category: Lu, Y.]] | [[Category: Lu, Y.]] | ||
[[Category: McCracken, S.]] | [[Category: McCracken, S.]] | ||
[[Category: Pineda-Lucena, A.]] | [[Category: Pineda-Lucena, A.]] | ||
| - | [[Category: Szymczyna, B | + | [[Category: Szymczyna, B R.]] |
[[Category: four helix bundle]] | [[Category: four helix bundle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:57:31 2008'' |
Revision as of 11:57, 21 February 2008
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Solution structure of the PWI motif from SRm160
Overview
The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids. Deletion of the motif prevents SRm160 from binding RNA and stimulating 3'-end cleavage, and its substitution with a heterologous RNA-binding domain restores these functions. The NMR solution structure of the SRm160-PWI motif reveals a novel, four-helix bundle and represents the first example of an alpha-helical fold that can bind single-stranded (ss)RNA. Structure-guided mutagenesis indicates that the same surface is involved in RNA and DNA binding and requires the cooperative action of a highly conserved, adjacent basic region. Thus, the PWI motif is a novel type of nucleic acid-binding domain that likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3'-end formation.
About this Structure
1MP1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing., Szymczyna BR, Bowman J, McCracken S, Pineda-Lucena A, Lu Y, Cox B, Lambermon M, Graveley BR, Arrowsmith CH, Blencowe BJ, Genes Dev. 2003 Feb 15;17(4):461-75. PMID:12600940
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