1mkh

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{{STRUCTURE_1mkh| PDB=1mkh | SCENE= }}
{{STRUCTURE_1mkh| PDB=1mkh | SCENE= }}
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'''C-terminal domain of methionyl-tRNA synthetase from Pyrococcus abyssi'''
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===C-terminal domain of methionyl-tRNA synthetase from Pyrococcus abyssi===
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==Overview==
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The minimal polypeptide supporting full methionyl-tRNA synthetase (MetRS) activity is composed of four domains: a catalytic Rossmann fold, a connective peptide, a KMSKS domain, and a C-terminal alpha helix bundle domain. The minimal MetRS behaves as a monomer. In several species, MetRS is a homodimer because of a C-terminal domain appended to the core polypeptide. Upon truncation of this C-terminal domain, subunits dissociate irreversibly. Here, the C-terminal domain of dimeric MetRS from Pyrococcus abyssi was isolated and studied. It displays nonspecific tRNA-binding properties and has a crystalline structure closely resembling that of Trbp111, a dimeric tRNA-binding protein found in many bacteria and archaea. The obtained 3D model was used to direct mutations against dimerization of Escherichia coli MetRS. Comparison of the resulting mutants to native and C-truncated MetRS shows that the presence of the appended C-domain improves tRNA(Met) binding affinity. However, dimer formation is required to evidence the gain in affinity.
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(as it appears on PubMed at http://www.pubmed.gov), where 12390027 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12390027}}
==About this Structure==
==About this Structure==
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[[Category: Beta barrel]]
[[Category: Beta barrel]]
[[Category: Dimerization domain]]
[[Category: Dimerization domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:17:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:11:08 2008''

Revision as of 21:11, 2 July 2008

Image:1mkh.png

Template:STRUCTURE 1mkh

C-terminal domain of methionyl-tRNA synthetase from Pyrococcus abyssi

Template:ABSTRACT PUBMED 12390027

About this Structure

1MKH is a Single protein structure of sequence from Pyrococcus abyssi. Full crystallographic information is available from OCA.

Reference

Structure and function of the C-terminal domain of methionyl-tRNA synthetase., Crepin T, Schmitt E, Blanquet S, Mechulam Y, Biochemistry. 2002 Oct 29;41(43):13003-11. PMID:12390027

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