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1mq2
From Proteopedia
(New page: 200px<br /> <applet load="1mq2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mq2, resolution 3.1Å" /> '''Human DNA Polymerase...) |
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| - | [[Image:1mq2.gif|left|200px]]<br /> | + | [[Image:1mq2.gif|left|200px]]<br /><applet load="1mq2" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1mq2" size=" | + | |
caption="1mq2, resolution 3.1Å" /> | caption="1mq2, resolution 3.1Å" /> | ||
'''Human DNA Polymerase Beta Complexed With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine and dAMP'''<br /> | '''Human DNA Polymerase Beta Complexed With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine and dAMP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Oxidative damage to DNA generates 8-oxo-7,8-dihydro-2'-deoxyguanosine | + | Oxidative damage to DNA generates 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG). During DNA replication and repair synthesis, 8-oxodG can pair with cytosine or adenine. The ability to accurately replicate through this lesion depends on the DNA polymerase. We report the first structure of a polymerase with a promutagenic DNA lesion, 8-oxodG, in the confines of its active site. The modified guanine residue is in an anti conformation and forms Watson-Crick hydrogen bonds with an incoming dCTP. To accommodate the oxygen at C8, the 5'-phosphate backbone of the templating nucleotide flips 180 degrees. Thus, the flexibility of the template sugar-phosphate backbone near the polymerase active site is one parameter that influences the anti-syn equilibrium of 8-oxodG. Our results provide insights into the mechanisms employed by polymerases to select the complementary dNTP. |
==About this Structure== | ==About this Structure== | ||
| - | 1MQ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NA and DA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | + | 1MQ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=DA:'>DA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQ2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Beard, W | + | [[Category: Beard, W A.]] |
| - | [[Category: Grollman, A | + | [[Category: Grollman, A P.]] |
| - | [[Category: Krahn, J | + | [[Category: Krahn, J M.]] |
[[Category: Miller, H.]] | [[Category: Miller, H.]] | ||
| - | [[Category: Wilson, S | + | [[Category: Wilson, S H.]] |
[[Category: DA]] | [[Category: DA]] | ||
[[Category: NA]] | [[Category: NA]] | ||
| Line 25: | Line 24: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:57:49 2008'' |
Revision as of 11:57, 21 February 2008
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Human DNA Polymerase Beta Complexed With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine and dAMP
Overview
Oxidative damage to DNA generates 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG). During DNA replication and repair synthesis, 8-oxodG can pair with cytosine or adenine. The ability to accurately replicate through this lesion depends on the DNA polymerase. We report the first structure of a polymerase with a promutagenic DNA lesion, 8-oxodG, in the confines of its active site. The modified guanine residue is in an anti conformation and forms Watson-Crick hydrogen bonds with an incoming dCTP. To accommodate the oxygen at C8, the 5'-phosphate backbone of the templating nucleotide flips 180 degrees. Thus, the flexibility of the template sugar-phosphate backbone near the polymerase active site is one parameter that influences the anti-syn equilibrium of 8-oxodG. Our results provide insights into the mechanisms employed by polymerases to select the complementary dNTP.
About this Structure
1MQ2 is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Structure of DNA polymerase beta with the mutagenic DNA lesion 8-oxodeoxyguanine reveals structural insights into its coding potential., Krahn JM, Beard WA, Miller H, Grollman AP, Wilson SH, Structure. 2003 Jan;11(1):121-7. PMID:12517346
Page seeded by OCA on Thu Feb 21 13:57:49 2008
Categories: DNA-directed DNA polymerase | Homo sapiens | Single protein | Beard, W A. | Grollman, A P. | Krahn, J M. | Miller, H. | Wilson, S H. | DA | NA | Dna | Transferase
