1ml6

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{{STRUCTURE_1ml6| PDB=1ml6 | SCENE= }}
{{STRUCTURE_1ml6| PDB=1ml6 | SCENE= }}
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'''Crystal Structure of mGSTA2-2 in Complex with the Glutathione Conjugate of Benzo[a]pyrene-7(R),8(S)-Diol-9(S),10(R)-Epoxide'''
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===Crystal Structure of mGSTA2-2 in Complex with the Glutathione Conjugate of Benzo[a]pyrene-7(R),8(S)-Diol-9(S),10(R)-Epoxide===
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==Overview==
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Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues differ between the sequences of mGSTA1-1 and 2-2. However, the catalytic efficiency of mGSTA1-1 for GSH conjugation of (+)-anti-BPDE is &gt;3-fold higher as compared with mGSTA2-2. The crystal structure of mGSTA1-1 in complex with the GSH conjugate of (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (GSBpd) reveals that R216 and I221 in the last helix play important roles in catalysis [Gu, Y., Singh, S. V., and Ji, X. (2000) Biochemistry 39, 12552-12557]. The crystal structure of mGSTA2-2 in complex with GSBpd has been determined, which reveals a different binding mode of GSBpd. Comparison of the two structures suggests that residues 207 and 221 are responsible for the different binding mode of GSBpd and therefore contribute to the distinct catalytic efficiency of the two isozymes.
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{{ABSTRACT_PUBMED_12549910}}
==About this Structure==
==About this Structure==
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[[Category: Gst detoxification]]
[[Category: Gst detoxification]]
[[Category: Mgsta2-2]]
[[Category: Mgsta2-2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:21:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:14:46 2008''

Revision as of 21:14, 2 July 2008

Image:1ml6.png

Template:STRUCTURE 1ml6

Crystal Structure of mGSTA2-2 in Complex with the Glutathione Conjugate of Benzo[a]pyrene-7(R),8(S)-Diol-9(S),10(R)-Epoxide

Template:ABSTRACT PUBMED 12549910

About this Structure

1ML6 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Residues 207, 216, and 221 and the catalytic activity of mGSTA1-1 and mGSTA2-2 toward benzo[a]pyrene-(7R,8S)-diol-(9S,10R)-epoxide., Gu Y, Xiao B, Wargo HL, Bucher MH, Singh SV, Ji X, Biochemistry. 2003 Feb 4;42(4):917-21. PMID:12549910

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