1mlx

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{{STRUCTURE_1mlx| PDB=1mlx | SCENE= }}
{{STRUCTURE_1mlx| PDB=1mlx | SCENE= }}
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'''Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex'''
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===Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex===
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==Overview==
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A novel 2'-modification, 2'-O-[2-(methylthio)ethyl] or 2'-O-MTE, has been incorporated into oligonucleotides and evaluated for properties relevant to antisense activity. The results were compared with the previously characterized 2'-O-[2-(methoxy)ethyl] 2'-O-MOE modification. As expected, the 2'-O-MTE modified oligonucleotides exhibited improved binding to human serum albumin compared to the 2'-O-MOE modified oligonucleotides. The 2'-O-MTE oligonucleotides maintained high binding affinity to target RNA. Nuclease digestion of 2'-O-MTE oligonucleotides showed that they have limited resistance to exonuclease degradation. We analyzed the crystal structure of a decamer DNA duplex containing the 2'-O-MTE modifcation. Analysis of the crystal structure provides insight into the improved RNA binding affinity, protein binding affinity and limited resistance of 2'-O-MTE modified oligonucleotides to exonuclease degradation.
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{{ABSTRACT_PUBMED_12269806}}
==About this Structure==
==About this Structure==
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[[Category: Protein binding affinity]]
[[Category: Protein binding affinity]]
[[Category: Rna affinity]]
[[Category: Rna affinity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:23:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:17:52 2008''

Revision as of 21:17, 2 July 2008

Image:1mlx.png

Template:STRUCTURE 1mlx

Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex

Template:ABSTRACT PUBMED 12269806

About this Structure

Full crystallographic information is available from OCA.

Reference

2'-O-[2-(methylthio)ethyl]-modified oligonucleotide: an analogue of 2'-O-[2-(methoxy)-ethyl]-modified oligonucleotide with improved protein binding properties and high binding affinity to target RNA., Prakash TP, Manoharan M, Kawasaki AM, Fraser AS, Lesnik EA, Sioufi N, Leeds JM, Teplova M, Egli M, Biochemistry. 2002 Oct 1;41(39):11642-8. PMID:12269806

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