We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1mnv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1mnv.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1mnv.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1mnv| PDB=1mnv | SCENE= }}
{{STRUCTURE_1mnv| PDB=1mnv | SCENE= }}
-
'''Actinomycin D binding to ATGCTGCAT'''
+
===Actinomycin D binding to ATGCTGCAT===
-
==Overview==
+
<!--
-
The potent anticancer drug actinomycin D (ActD) acts by binding to DNA GpC sequences, thereby interfering with essential biological processes including replication, transcription and topoisomerase. Certain neurological diseases are correlated with expansion of (CTG)n trinucleotide sequences, which contain many contiguous GpC sites separated by a single base pair. In order to characterize the binding of ActD to CTG triplet repeat sequences, we carried out heat denaturation and CD analyses, which showed that adjacent GpC sequences flanking a T:T mismatch are preferred ActD-binding sites, and that ActD binding results in a conformational transition to A-type structure. The structural basis of the strong binding of ActD to neighboring GpC sites flanking a T:T mismatch was provided by the crystal structure of ActD bound to ATGCTGCAT, which contains a CTG triplet sequence. Binding of two ActD molecules to GCTGC causes a kink in the DNA helix. In addition, using a synthetic self-priming DNA model, 5'-(CAG)4(CTG)(16)-3', we observed that ActD can trap the cruciform or duplexes of (CTG)n and interfere with the expansion process of CTG triplet repeats as shown by gel electrophoretic expansion assay. Our results may provide the possible biological consequence of ActD bound to CTG triplet repeat sequences.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12433994}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12433994 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12433994}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Neurological disease]]
[[Category: Neurological disease]]
[[Category: X-ray diffraction]]
[[Category: X-ray diffraction]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:28:45 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:30:24 2008''

Revision as of 21:30, 2 July 2008

Image:1mnv.png

Template:STRUCTURE 1mnv

Actinomycin D binding to ATGCTGCAT

Template:ABSTRACT PUBMED 12433994

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of actinomycin D bound to the CTG triplet repeat sequences linked to neurological diseases., Hou MH, Robinson H, Gao YG, Wang AH, Nucleic Acids Res. 2002 Nov 15;30(22):4910-7. PMID:12433994

Page seeded by OCA on Thu Jul 3 00:30:24 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mnv"
Personal tools