1mx3
From Proteopedia
(New page: 200px<br /> <applet load="1mx3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mx3, resolution 1.95Å" /> '''Crystal structure o...) |
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| - | [[Image:1mx3.gif|left|200px]]<br /> | + | [[Image:1mx3.gif|left|200px]]<br /><applet load="1mx3" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1mx3" size=" | + | |
caption="1mx3, resolution 1.95Å" /> | caption="1mx3, resolution 1.95Å" /> | ||
'''Crystal structure of CtBP dehydrogenase core holo form'''<br /> | '''Crystal structure of CtBP dehydrogenase core holo form'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Transcriptional repression is based on the selective actions of recruited | + | Transcriptional repression is based on the selective actions of recruited corepressor complexes, including those with enzymatic activities. One well-characterized developmentally important corepressor is the C-terminal binding protein (CtBP). Although intriguingly related in sequence to D2 hydroxyacid dehydrogenases, the mechanism by which CtBP functions remains unclear. We report here biochemical and crystallographic studies which reveal that CtBP is a functional dehydrogenase. In addition, both a cofactor-dependent conformational change, with NAD(+) and NADH being equivalently effective, and the active site residues are linked to the binding of the PXDLS consensus recognition motif on repressors, such as E1A and RIP140. Together, our data suggest that CtBP is an NAD(+)-regulated component of critical complexes for specific repression events in cells. |
==About this Structure== | ==About this Structure== | ||
| - | 1MX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAD and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1MX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAD:'>NAD</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MX3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Aggarwal, A | + | [[Category: Aggarwal, A K.]] |
| - | [[Category: Carlson, J | + | [[Category: Carlson, J E.]] |
| - | [[Category: Edwards, T | + | [[Category: Edwards, T E.]] |
| - | [[Category: Escalante, C | + | [[Category: Escalante, C R.]] |
[[Category: Kumar, V.]] | [[Category: Kumar, V.]] | ||
| - | [[Category: Ohgi, K | + | [[Category: Ohgi, K E.]] |
| - | [[Category: Rose, D | + | [[Category: Rose, D W.]] |
[[Category: ACY]] | [[Category: ACY]] | ||
[[Category: NAD]] | [[Category: NAD]] | ||
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[[Category: transcriptional corepressor]] | [[Category: transcriptional corepressor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:59:56 2008'' |
Revision as of 11:59, 21 February 2008
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Crystal structure of CtBP dehydrogenase core holo form
Overview
Transcriptional repression is based on the selective actions of recruited corepressor complexes, including those with enzymatic activities. One well-characterized developmentally important corepressor is the C-terminal binding protein (CtBP). Although intriguingly related in sequence to D2 hydroxyacid dehydrogenases, the mechanism by which CtBP functions remains unclear. We report here biochemical and crystallographic studies which reveal that CtBP is a functional dehydrogenase. In addition, both a cofactor-dependent conformational change, with NAD(+) and NADH being equivalently effective, and the active site residues are linked to the binding of the PXDLS consensus recognition motif on repressors, such as E1A and RIP140. Together, our data suggest that CtBP is an NAD(+)-regulated component of critical complexes for specific repression events in cells.
About this Structure
1MX3 is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Transcription corepressor CtBP is an NAD(+)-regulated dehydrogenase., Kumar V, Carlson JE, Ohgi KA, Edwards TA, Rose DW, Escalante CR, Rosenfeld MG, Aggarwal AK, Mol Cell. 2002 Oct;10(4):857-69. PMID:12419229
Page seeded by OCA on Thu Feb 21 13:59:56 2008
