1n3y
From Proteopedia
(New page: 200px<br /> <applet load="1n3y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n3y, resolution 1.65Å" /> '''Crystal structure o...) |
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caption="1n3y, resolution 1.65Å" /> | caption="1n3y, resolution 1.65Å" /> | ||
'''Crystal structure of the alpha-X beta2 integrin I domain'''<br /> | '''Crystal structure of the alpha-X beta2 integrin I domain'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1N3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1N3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: alpha/beta rossmann fold]] | [[Category: alpha/beta rossmann fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:26:57 2008'' |
Revision as of 14:26, 15 February 2008
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Crystal structure of the alpha-X beta2 integrin I domain
Overview
The integrin alpha X beta 2 (CD11c/CD18, p150,95) binds ligands through, the I domain of the alpha X subunit. Ligands include the complement factor, fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on, other leukocytes, suggests a role in the immune response. We now report, the structure of the alpha X I domain resolved at 1.65 A by x-ray, crystallography. To analyze structural requirements for ligand binding we, made a mutation in the alpha X I domain C-terminal helix, which increased, the affinity for iC3b approximately 200-fold to 2.4 microM compared with, the wild-type domain affinity of approximately 400 microM. Gel permeation, chromatography supported a conformational change between the wild-type and, mutated domains. Conservation of allosteric regulation in the alpha X I, domain points to the functional importance of this phenomenon.
About this Structure
1N3Y is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and allosteric regulation of the alpha X beta 2 integrin I domain., Vorup-Jensen T, Ostermeier C, Shimaoka M, Hommel U, Springer TA, Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1873-8. Epub 2003 Jan 28. PMID:12554829
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