1mtx

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{{STRUCTURE_1mtx| PDB=1mtx | SCENE= }}
{{STRUCTURE_1mtx| PDB=1mtx | SCENE= }}
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'''DETERMINATION OF THE THREE-DIMENSIONAL STRUCTURE OF MARGATOXIN BY 1H, 13C, 15N TRIPLE-RESONANCE NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY'''
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===DETERMINATION OF THE THREE-DIMENSIONAL STRUCTURE OF MARGATOXIN BY 1H, 13C, 15N TRIPLE-RESONANCE NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY===
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==Overview==
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The solution structure of the 39-residue peptide margatoxin, a scorpion toxin that selectively blocks the voltage-gated potassium-channel Kv1.3, has been determined by NMR spectroscopy. The toxin was isotopically labeled with 13C and 15N and studied using two-dimensional homonuclear and three- and four-dimensional heteronuclear NMR spectroscopy. The final structure was determined using 501 constraints, comprising 422 NOE constraints, 60 dihedral angle constraints, 9 disulfide constraints, and 10 hydrogen bond constraints. Structures were initially determined with the program PEGASUS and subsequently refined with X-PLOR. The average rms deviation from a calculated average structure for the backbone atoms of residues 3-38 is 0.40 A. A helix is present from residues 11 to 20 and includes two proline residues at positions 15 and 16. A loop at residues 21-24 leads into a two-strand antiparallel sheet from residues 25 to 38 with a turn at residues 30-33. Residues 3-6 run adjacent to the 33-38 strand but do not form a canonical beta-strand. The two additional residues of margatoxin, relative to the related toxins charybdotoxin and iberiotoxin, insert in a manner that extends the beta-sheet by one residue. Otherwise, the global structure is very similar to that of these two other toxins. The longer sheet may have implications for channel selectivity.
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{{ABSTRACT_PUBMED_7999764}}
==About this Structure==
==About this Structure==
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1MTX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_margaritatus Centruroides margaritatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTX OCA].
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1MTX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_margaritatus Centruroides margaritatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTX OCA].
==Reference==
==Reference==
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[[Category: Williamson, J M.]]
[[Category: Williamson, J M.]]
[[Category: Toxin]]
[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:42:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:09:21 2008''

Revision as of 05:09, 29 July 2008

Image:1mtx.png

Template:STRUCTURE 1mtx

DETERMINATION OF THE THREE-DIMENSIONAL STRUCTURE OF MARGATOXIN BY 1H, 13C, 15N TRIPLE-RESONANCE NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Template:ABSTRACT PUBMED 7999764

About this Structure

1MTX is a Single protein structure of sequence from Centruroides margaritatus. Full experimental information is available from OCA.

Reference

Determination of the three-dimensional structure of margatoxin by 1H, 13C, 15N triple-resonance nuclear magnetic resonance spectroscopy., Johnson BA, Stevens SP, Williamson JM, Biochemistry. 1994 Dec 20;33(50):15061-70. PMID:7999764

Page seeded by OCA on Tue Jul 29 08:09:21 2008

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