8kbw
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The crystal structure of syn-copalyl diphosphate synthase from Oryza sativa== |
| + | <StructureSection load='8kbw' size='340' side='right'caption='[[8kbw]], [[Resolution|resolution]] 3.49Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8kbw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8KBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8KBW FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.49Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8kbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8kbw OCA], [https://pdbe.org/8kbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8kbw RCSB], [https://www.ebi.ac.uk/pdbsum/8kbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8kbw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CPS4_ORYSJ CPS4_ORYSJ] Catalyzes the conversion of geranylgeranyl diphosphate to the phytoalexin precursor syn-copalyl diphosphate (PubMed:15341631, PubMed:15255861, PubMed:23621683). Required for the biosynthesis of momilactones that exude from roots and act as allelochemicals against lowland weeds in paddy soil (PubMed:23621683).<ref>PMID:15255861</ref> <ref>PMID:15341631</ref> <ref>PMID:23621683</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The large superfamily of labdane-related diterpenoids is defined by the cyclization of linear geranylgeranyl pyrophosphate (GGPP), catalyzed by copalyl diphosphate synthases (CPSs) to form the basic decalin core, the copalyl diphosphates (CPPs). Three stereochemically distinct CPPs have been found in plants, namely (+)-CPP, ent-CPP and syn-CPP. Here, we used X-ray crystallography and cryo-EM methods to describe different oligomeric structures of a syn-copalyl diphosphate synthase from Oryza sativa (OsCyc1), and provided a cryo-EM structure of OsCyc1(D367A) mutant in complex with the substrate GGPP. Further analysis showed that tetramers are the dominant form of OsCyc1 in solution and are not necessary for enzyme activity in vitro. Through rational design, we identified an OsCyc1 mutant that can generate ent-CPP in addition to syn-CPP. Our work provides a structural and mechanistic basis for comparing different CPSs and paves the way for further enzyme design to obtain diterpene derivatives with specific chirality. | ||
| - | + | Structural and functional investigations of syn-copalyl diphosphate synthase from Oryza sativa.,Ma X, Xu H, Tong Y, Luo Y, Dong Q, Jiang T Commun Chem. 2023 Nov 6;6(1):240. doi: 10.1038/s42004-023-01042-w. PMID:37932442<ref>PMID:37932442</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8kbw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Oryza sativa Japonica Group]] | ||
| + | [[Category: Jiang T]] | ||
| + | [[Category: Ma XL]] | ||
| + | [[Category: Xu HF]] | ||
Current revision
The crystal structure of syn-copalyl diphosphate synthase from Oryza sativa
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