8tv8
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of nontypeable Haemophilus influenzae SapA== | |
| + | <StructureSection load='8tv8' size='340' side='right'caption='[[8tv8]], [[Resolution|resolution]] 2.25Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8tv8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae_86-028NP Haemophilus influenzae 86-028NP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TV8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tv8 OCA], [https://pdbe.org/8tv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tv8 RCSB], [https://www.ebi.ac.uk/pdbsum/8tv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tv8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q4QL73_HAEI8 Q4QL73_HAEI8] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with respiratory diseases, including otitis media and exacerbations of chronic obstructive pulmonary disease. NTHi exhibits resistance to killing by host antimicrobial peptides (AMPs) mediated by SapA, the substrate binding protein of the sensitivity to antimicrobial peptides (Sap) transporter. However, the specific mechanisms by which SapA selectively binds various AMPs such as defensins and cathelicidin are unknown. In this study, we report mutational analyses of both defensin AMPs and the SapA binding pocket to define the specificity of AMP recognition. Bactericidal assays revealed that NTHi lacking SapA are more susceptible to human beta defensins and LL-37, while remaining highly resistant to a human alpha defensin. In contrast to homologues, our research underscores the distinct specificity of NTHi SapA, which selectively recognizes and binds to peptides containing the charged-hydrophobic motif PKE and RRY. These findings provide valuable insight into the divergence of SapA among bacterial species and NTHi SapA's ability to selectively interact with specific AMPs to mediate resistance. | ||
| - | + | Antimicrobial Peptide Recognition Motif of the Substrate Binding Protein SapA from Nontypeable Haemophilus influenzae.,Rivera KG, Tanaka KJ, Buechel ER, Origel O Jr, Harrison A, Mason KM, Pinkett HW Biochemistry. 2024 Jan 8. doi: 10.1021/acs.biochem.3c00562. PMID:38189237<ref>PMID:38189237</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8tv8" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Haemophilus influenzae 86-028NP]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Buechel ER]] | ||
| + | [[Category: Pinkett HW]] | ||
| + | [[Category: Rivera KG]] | ||
| + | [[Category: Tanaka KJ]] | ||
Revision as of 10:10, 17 January 2024
Crystal structure of nontypeable Haemophilus influenzae SapA
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