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1mvz

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[[Image:1mvz.gif|left|200px]]
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{{Seed}}
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[[Image:1mvz.png|left|200px]]
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{{STRUCTURE_1mvz| PDB=1mvz | SCENE= }}
{{STRUCTURE_1mvz| PDB=1mvz | SCENE= }}
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'''NMR solution structure of a Bowman Birk inhibitor isolated from snail medic seeds (Medicago Scutellata)'''
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===NMR solution structure of a Bowman Birk inhibitor isolated from snail medic seeds (Medicago Scutellata)===
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==Overview==
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The high-resolution three-dimensional structure of a Bowman Birk inhibitor, purified from snail medic seeds (Medicago scutellata) (MSTI), has been determined in solution by 1H NMR spectroscopy at pH 5.6 and 27 degrees C. The structure of MSTI comprises two distinct symmetric domains each composed of a three-stranded beta-sheet containing a VIb type loop, where the active sites are located. A characteristic geometry of three aromatic residues confers stability to this protein, and we observe that this feature is conserved in all the Bowman Birk inhibitors of known structure. The two active domains exhibit different conformational features: the second domain displays higher flexibility and hydrophobicity with respect to the first one, and these properties have been correlated to a lower trypsin inhibitory specificity, in agreement with titration studies that have shown a stoichiometric ratio MSTI:trypsin of 1:1.5. NMR analysis indicated that MSTI undergoes self-association at concentrations higher than 2 mM, and the residues involved in this mechanism are localized at opposite faces of the molecule, having the highest positive and negative potential, respectively, thus indicating that electrostatic intermolecular interactions are the driving forces for MSTI association. Most of the residues affected by self-association are highly conserved in BBIs from different seeds, suggesting a functional relevance for these charged superficial patches, possibly involved in the interaction with other enzymes or macromolecules, thus triggering anti-carcinogenic activity.
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(as it appears on PubMed at http://www.pubmed.gov), where 12627949 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12627949}}
==About this Structure==
==About this Structure==
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1MVZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_scutellata Medicago scutellata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVZ OCA].
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1MVZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_scutellata Medicago scutellata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVZ OCA].
==Reference==
==Reference==
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[[Category: Three stranded beta-sheet]]
[[Category: Three stranded beta-sheet]]
[[Category: Vib type turn]]
[[Category: Vib type turn]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:47:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:18:45 2008''

Revision as of 16:18, 27 July 2008

Image:1mvz.png

Template:STRUCTURE 1mvz

NMR solution structure of a Bowman Birk inhibitor isolated from snail medic seeds (Medicago Scutellata)

Template:ABSTRACT PUBMED 12627949

About this Structure

1MVZ is a Single protein structure of sequence from Medicago scutellata. Full experimental information is available from OCA.

Reference

Anticarcinogenic Bowman Birk inhibitor isolated from snail medic seeds (Medicago scutellata): solution structure and analysis of self-association behavior., Catalano M, Ragona L, Molinari H, Tava A, Zetta L, Biochemistry. 2003 Mar 18;42(10):2836-46. PMID:12627949

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