This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1mwj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1mwj.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1mwj.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1mwj| PDB=1mwj | SCENE= }}
{{STRUCTURE_1mwj| PDB=1mwj | SCENE= }}
-
'''Crystal Structure of a MUG-DNA pseudo substrate complex'''
+
===Crystal Structure of a MUG-DNA pseudo substrate complex===
-
==Overview==
+
<!--
-
The bacterial mismatch-specific uracil-DNA glycosylase (MUG) and eukaryotic thymine-DNA glycosylase (TDG) enzymes form a homologous family of DNA glycosylases that initiate base-excision repair of G:U/T mismatches. Despite low sequence homology, the MUG/TDG enzymes are structurally related to the uracil-DNA glycosylase enzymes, but have a very different mechanism for substrate recognition. We have now determined the crystal structure of the Escherichia coli MUG enzyme complexed with an oligonucleotide containing a non-hydrolysable deoxyuridine analogue mismatched with guanine, providing the first structure of an intact substrate-nucleotide productively bound to a hydrolytic DNA glycosylase. The structure of this complex explains the preference for G:U over G:T mispairs, and reveals an essentially non-specific pyrimidine-binding pocket that allows MUG/TDG enzymes to excise the alkylated base, 3, N(4)-ethenocytosine. Together with structures for the free enzyme and for an abasic-DNA product complex, the MUG-substrate analogue complex reveals the conformational changes accompanying the catalytic cycle of substrate binding, base excision and product release.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10581234}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10581234 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10581234}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
[[Category: Uracil recognition.]]
[[Category: Uracil recognition.]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:48:21 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:51:45 2008''

Revision as of 14:51, 27 July 2008

Image:1mwj.png

Template:STRUCTURE 1mwj

Crystal Structure of a MUG-DNA pseudo substrate complex

Template:ABSTRACT PUBMED 10581234

About this Structure

1MWJ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of a thwarted mismatch glycosylase DNA repair complex., Barrett TE, Scharer OD, Savva R, Brown T, Jiricny J, Verdine GL, Pearl LH, EMBO J. 1999 Dec 1;18(23):6599-609. PMID:10581234

Page seeded by OCA on Sun Jul 27 17:51:45 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mwj"
Personal tools