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1mwk

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[[Image:1mwk.gif|left|200px]]
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{{Seed}}
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[[Image:1mwk.png|left|200px]]
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{{STRUCTURE_1mwk| PDB=1mwk | SCENE= }}
{{STRUCTURE_1mwk| PDB=1mwk | SCENE= }}
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'''ParM from plasmid R1 APO form'''
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===ParM from plasmid R1 APO form===
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==Overview==
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It was the general belief that DNA partitioning in prokaryotes is independent of a cytoskeletal structure, which in eukaryotic cells is indispensable for DNA segregation. Recently, however, immunofluorescence microscopy revealed highly dynamic, filamentous structures along the longitudinal axis of Escherichia coli formed by ParM, a plasmid-encoded protein required for accurate segregation of low-copy-number plasmid R1. We show here that ParM polymerizes into double helical protofilaments with a longitudinal repeat similar to filamentous actin (F-actin) and MreB filaments that maintain the cell shape of non-spherical bacteria. The crystal structure of ParM with and without ADP demonstrates that it is a member of the actin family of proteins and shows a domain movement of 25 degrees upon nucleotide binding. Furthermore, the crystal structure of ParM reveals major differences in the protofilament interface compared with F-actin, despite the similar arrangement of the subunits within the filaments. Thus, there is now evidence for cytoskeletal structures, formed by actin-like filaments that are involved in plasmid partitioning in E.coli.
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The line below this paragraph, {{ABSTRACT_PUBMED_12486014}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12486014 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12486014}}
==About this Structure==
==About this Structure==
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[[Category: Plasmid]]
[[Category: Plasmid]]
[[Category: Plasmid partition]]
[[Category: Plasmid partition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:48:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:12:00 2008''

Revision as of 12:12, 29 July 2008

Image:1mwk.png

Template:STRUCTURE 1mwk

ParM from plasmid R1 APO form

Template:ABSTRACT PUBMED 12486014

About this Structure

1MWK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

F-actin-like filaments formed by plasmid segregation protein ParM., van den Ent F, Moller-Jensen J, Amos LA, Gerdes K, Lowe J, EMBO J. 2002 Dec 16;21(24):6935-43. PMID:12486014

Page seeded by OCA on Tue Jul 29 15:12:00 2008

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