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1myw

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{{STRUCTURE_1myw| PDB=1myw | SCENE= }}
{{STRUCTURE_1myw| PDB=1myw | SCENE= }}
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'''CRYSTAL STRUCTURE OF A YELLOW FLUORESCENT PROTEIN WITH IMPROVED MATURATION AND REDUCED ENVIRONMENTAL SENSITIVITY'''
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===CRYSTAL STRUCTURE OF A YELLOW FLUORESCENT PROTEIN WITH IMPROVED MATURATION AND REDUCED ENVIRONMENTAL SENSITIVITY===
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==Overview==
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Yellow emission variants of green fluorescent protein (GFP) have been found useful in a variety of applications in biological systems due to their red-shifted emission spectrum and sensitivity to environmental parameters, such as pH and ionic strength. However, slow maturation properties and new requirements for more intense fluorescence necessitated further mutagenesis studies of these proteins. Venus, a new variant with improved maturation and brightness, as well as reduced environmental dependence, was recently developed by introducing five mutations into the well characterized variant, enhanced yellow fluorescent protein (EYFP). In this paper, we present the crystal structure of Venus at 2.2 A resolution, which enabled us to correlate its novel features with these mutation points. The rearrangement of several side chains near the chromophore, initiated by the F46L mutation, was found to improve maturation at 37 degrees C by removing steric and energetic constraints, which may hinder folding of the polypeptide chain, and by accelerating the oxidation of the Calpha-Cbeta bond of Tyr(66) during chromophore formation. M153T, V163A, and S175G were also found to improve the rate of maturation by creating regions of greater flexibility. F64L induced large conformational changes in the molecule, leading to the removal of halide sensitivity by preventing ion access to the binding site.
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(as it appears on PubMed at http://www.pubmed.gov), where 12370172 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12370172}}
==About this Structure==
==About this Structure==
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[[Category: Photoactive protein]]
[[Category: Photoactive protein]]
[[Category: Yellow-emission variant]]
[[Category: Yellow-emission variant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:52:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:17:53 2008''

Revision as of 04:18, 29 July 2008

Image:1myw.png

Template:STRUCTURE 1myw

CRYSTAL STRUCTURE OF A YELLOW FLUORESCENT PROTEIN WITH IMPROVED MATURATION AND REDUCED ENVIRONMENTAL SENSITIVITY

Template:ABSTRACT PUBMED 12370172

About this Structure

1MYW is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.

Reference

Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity., Rekas A, Alattia JR, Nagai T, Miyawaki A, Ikura M, J Biol Chem. 2002 Dec 27;277(52):50573-8. Epub 2002 Oct 4. PMID:12370172

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