We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1mzt

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1mzt.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1mzt.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1mzt| PDB=1mzt | SCENE= }}
{{STRUCTURE_1mzt| PDB=1mzt | SCENE= }}
-
'''NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes'''
+
===NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes===
-
==Overview==
+
<!--
-
A solid-state NMR approach for simultaneous resonance assignment and three-dimensional structure determination of a membrane protein in lipid bilayers is described. The approach is based on the scattering, hence the descriptor "shotgun," of (15)N-labeled amino acids throughout the protein sequence (and the resulting NMR spectra). The samples are obtained by protein expression in bacteria grown on media in which one type of amino acid is labeled and the others are not. Shotgun NMR short-circuits the laborious and time-consuming process of obtaining complete sequential assignments prior to the calculation of a protein structure from the NMR data by taking advantage of the orientational information inherent to the spectra of aligned proteins. As a result, it is possible to simultaneously assign resonances and measure orientational restraints for structure determination. A total of five two-dimensional (1)H/(15)N PISEMA (polarization inversion spin exchange at the magic angle) spectra, from one uniformly and four selectively (15)N-labeled samples, were sufficient to determine the structure of the membrane-bound form of the 50-residue major pVIII coat protein of fd filamentous bacteriophage. Pisa (polarity index slat angle) wheels are an essential element in the process, which starts with the simultaneous assignment of resonances and the assembly of isolated polypeptide segments, and culminates in the complete three-dimensional structure of the protein with atomic resolution. The principles are also applicable to weakly aligned proteins studied by solution NMR spectroscopy. [The structure we determined for the membrane-bound form of the Fd bacteriophage pVIII coat protein has been deposited in the Protein Data Bank as PDB file 1MZT.]
+
The line below this paragraph, {{ABSTRACT_PUBMED_12592011}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12592011 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12592011}}
==About this Structure==
==About this Structure==
-
1MZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA].
+
1MZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA].
==Reference==
==Reference==
Line 27: Line 31:
[[Category: Membrane-bound]]
[[Category: Membrane-bound]]
[[Category: Pviii]]
[[Category: Pviii]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:54:45 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:11:29 2008''

Revision as of 20:11, 28 July 2008

Image:1mzt.png

Template:STRUCTURE 1mzt

NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes

Template:ABSTRACT PUBMED 12592011

About this Structure

1MZT is a Single protein structure of sequence from Filamentous phage. Full experimental information is available from OCA.

Reference

Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints., Marassi FM, Opella SJ, Protein Sci. 2003 Mar;12(3):403-11. PMID:12592011

Page seeded by OCA on Mon Jul 28 23:11:29 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mzt"
Personal tools