1n02

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1n02.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1n02.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1n02| PDB=1n02 | SCENE= }}
{{STRUCTURE_1n02| PDB=1n02 | SCENE= }}
-
'''Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N'''
+
===Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N===
-
==Overview==
+
<!--
-
The high-resolution solution structure of a monomeric circular permuted (cp) variant of the potent HIV-inactivating protein cyanovirin-N (CV-N) was determined by NMR. Comparison with the wild-type (wt) structure revealed that the observed loss in stability of cpCV-N compared to the wt protein is due to less favorable packing of several residues at the pseudo twofold axis that are responsible for holding the two halves of the molecule together. In particular, the N and C-terminal amino acid residues exhibit conformational flexibility, resulting in fewer and less favorable contacts between them. The important hydrophobic and hydrogen-bonding network between residues W49, D89, H90, Y100 and E101 that was observed in wt CV-N is no longer present. For instance, Y100 and E101 are flexible and the tryptophan side-chain is in a different conformation compared to the wt protein. The stability loss amounts to approximately 2kcal/mol and the mobility of the protein is evident by fast amide proton exchange throughout the chain. Mutation of the single proline residue to glycine (P52G) did not substantially affect the stability of the protein, in contrast to the finding for wtCV-N. The binding of high-mannose type oligosaccharides to cpCV-N was also investigated. Similar to wtCV-N, two carbohydrate-binding sites were identified on the protein and the Man alpha1--&gt;2Man linked moieties on the sugar were delineated as binding epitopes. Unlike in wtCV-N, the binding sites on cpCV-N are structurally similar and exhibit comparable binding affinities for the respective sugars. On the basis of the studies presented here and previous results on high-mannose binding to wtCV-N, we discuss a model for the interaction between gp120 and CV-N.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12473463}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12473463 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12473463}}
==About this Structure==
==About this Structure==
-
1N02 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA].
+
1N02 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N02 OCA].
==Reference==
==Reference==
Line 25: Line 29:
[[Category: Gronenborn, A M.]]
[[Category: Gronenborn, A M.]]
[[Category: Virus/viral protein inhibitor]]
[[Category: Virus/viral protein inhibitor]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:55:16 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:43:50 2008''

Revision as of 10:43, 29 July 2008

Image:1n02.png

Template:STRUCTURE 1n02

Solution Structure of a Circular-Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N

Template:ABSTRACT PUBMED 12473463

About this Structure

1N02 is a Single protein structure of sequence from Nostoc ellipsosporum. Full experimental information is available from OCA.

Reference

Solution structure of a circular-permuted variant of the potent HIV-inactivating protein cyanovirin-N: structural basis for protein stability and oligosaccharide interaction., Barrientos LG, Louis JM, Ratner DM, Seeberger PH, Gronenborn AM, J Mol Biol. 2003 Jan 3;325(1):211-23. PMID:12473463

Page seeded by OCA on Tue Jul 29 13:43:50 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n02"
Personal tools