8tw3
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==hMPV fusion protein complexed with single domain antibodies sdHMPV16 and sdHMPV12== | |
| + | <StructureSection load='8tw3' size='340' side='right'caption='[[8tw3]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8tw3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_metapneumovirus_A Human metapneumovirus A] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TW3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tw3 OCA], [https://pdbe.org/8tw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tw3 RCSB], [https://www.ebi.ac.uk/pdbsum/8tw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tw3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/H6X1Z0_9MONO H6X1Z0_9MONO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Human metapneumovirus (hMPV) is an important respiratory pathogen for which no licensed antivirals or vaccines exist. Single-domain antibodies represent promising antiviral biologics that can be easily produced and formatted. We describe the isolation and detailed characterization of two hMPV-neutralizing single-domain antibodies that are directed against the fusion protein F. One of these single-domain antibodies broadly neutralizes hMPV A and B strains, can prevent proteolytic maturation of F, and binds to an epitope in the F trimer interface. This suggests that hMPV pre-F undergoes trimer opening or "breathing" on infectious virions, exposing a vulnerable site for neutralizing antibodies. Finally, we show that this single-domain antibody, fused to a human IgG1 Fc, can protect cotton rats against hMPV replication, an important finding for potential future clinical applications. | ||
| - | + | A neutralizing single-domain antibody that targets the trimer interface of the human metapneumovirus fusion protein.,Ballegeer M, van Scherpenzeel RC, Delgado T, Iglesias-Caballero M, Garcia Barreno B, Pandey S, Rush SA, Kolkman JA, Mas V, McLellan JS, Saelens X mBio. 2024 Jan 16;15(1):e0212223. doi: 10.1128/mbio.02122-23. Epub 2023 Dec 20. PMID:38117059<ref>PMID:38117059</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8tw3" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Human metapneumovirus A]] | ||
| + | [[Category: Lama glama]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: McLellan JS]] | ||
| + | [[Category: Rush SA]] | ||
Revision as of 11:30, 24 January 2024
hMPV fusion protein complexed with single domain antibodies sdHMPV16 and sdHMPV12
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