1nf3
From Proteopedia
(New page: 200px<br /> <applet load="1nf3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nf3, resolution 2.10Å" /> '''Structure of Cdc42 ...) |
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| - | [[Image:1nf3.gif|left|200px]]<br /> | + | [[Image:1nf3.gif|left|200px]]<br /><applet load="1nf3" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1nf3" size=" | + | |
caption="1nf3, resolution 2.10Å" /> | caption="1nf3, resolution 2.10Å" /> | ||
'''Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6'''<br /> | '''Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cdc42 is a small GTPase that is required for cell polarity establishment | + | Cdc42 is a small GTPase that is required for cell polarity establishment in eukaryotes as diverse as budding yeast and mammals. Par6 is also implicated in metazoan cell polarity establishment and asymmetric cell divisions. Cdc42.GTP interacts with proteins that contain a conserved sequence called a CRIB motif. Uniquely, Par6 possesses a semi-CRIB motif that is not sufficient for binding to Cdc42. An adjacent PDZ domain is also necessary and is required for biological effects of Par6. Here we report the crystal structure of a complex between Cdc42 and the Par6 GTPase-binding domain. The semi-CRIB motif forms a beta-strand that inserts between the four strands of Cdc42 and the three strands of the PDZ domain to form a continuous eight-stranded sheet. Cdc42 induces a conformational change in Par6, detectable by fluorescence resonance energy transfer spectroscopy. Nuclear magnetic resonance studies indicate that the semi-CRIB motif of Par6 is at least partially structured by the PDZ domain. The structure highlights a novel role for a PDZ domain as a structural scaffold. |
==About this Structure== | ==About this Structure== | ||
| - | 1NF3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG and GNP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1NF3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GNP:'>GNP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Capaldo, C | + | [[Category: Capaldo, C T.]] |
[[Category: Gao, L.]] | [[Category: Gao, L.]] | ||
| - | [[Category: Garrard, S | + | [[Category: Garrard, S M.]] |
| - | [[Category: Macara, I | + | [[Category: Macara, I G.]] |
| - | [[Category: Rosen, M | + | [[Category: Rosen, M K.]] |
| - | [[Category: Tomchick, D | + | [[Category: Tomchick, D R.]] |
[[Category: GNP]] | [[Category: GNP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: switch i and ii]] | [[Category: switch i and ii]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:28 2008'' |
Revision as of 12:05, 21 February 2008
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Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6
Overview
Cdc42 is a small GTPase that is required for cell polarity establishment in eukaryotes as diverse as budding yeast and mammals. Par6 is also implicated in metazoan cell polarity establishment and asymmetric cell divisions. Cdc42.GTP interacts with proteins that contain a conserved sequence called a CRIB motif. Uniquely, Par6 possesses a semi-CRIB motif that is not sufficient for binding to Cdc42. An adjacent PDZ domain is also necessary and is required for biological effects of Par6. Here we report the crystal structure of a complex between Cdc42 and the Par6 GTPase-binding domain. The semi-CRIB motif forms a beta-strand that inserts between the four strands of Cdc42 and the three strands of the PDZ domain to form a continuous eight-stranded sheet. Cdc42 induces a conformational change in Par6, detectable by fluorescence resonance energy transfer spectroscopy. Nuclear magnetic resonance studies indicate that the semi-CRIB motif of Par6 is at least partially structured by the PDZ domain. The structure highlights a novel role for a PDZ domain as a structural scaffold.
About this Structure
1NF3 is a Protein complex structure of sequences from Homo sapiens and Mus musculus with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6., Garrard SM, Capaldo CT, Gao L, Rosen MK, Macara IG, Tomchick DR, EMBO J. 2003 Mar 3;22(5):1125-33. PMID:12606577
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