1nfi
From Proteopedia
(New page: 200px<br /> <applet load="1nfi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nfi, resolution 2.70Å" /> '''I-KAPPA-B-ALPHA/NF-...) |
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| - | [[Image:1nfi.gif|left|200px]]<br /> | + | [[Image:1nfi.gif|left|200px]]<br /><applet load="1nfi" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1nfi" size=" | + | |
caption="1nfi, resolution 2.70Å" /> | caption="1nfi, resolution 2.70Å" /> | ||
'''I-KAPPA-B-ALPHA/NF-KAPPA-B COMPLEX'''<br /> | '''I-KAPPA-B-ALPHA/NF-KAPPA-B COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The inhibitory protein, IkappaBalpha, sequesters the transcription factor, NF-kappaB, as an inactive complex in the cytoplasm. The structure of the | + | The inhibitory protein, IkappaBalpha, sequesters the transcription factor, NF-kappaB, as an inactive complex in the cytoplasm. The structure of the IkappaBalpha ankyrin repeat domain, bound to a partially truncated NF-kappaB heterodimer (p50/ p65), has been determined by X-ray crystallography at 2.7 A resolution. It shows a stack of six IkappaBalpha ankyrin repeats facing the C-terminal domains of the NF-kappaB Rel homology regions. Contacts occur in discontinuous patches, suggesting a combinatorial quality for ankyrin repeat specificity. The first two repeats cover an alpha helically ordered segment containing the p65 nuclear localization signal. The position of the sixth ankyrin repeat shows that full-length IkappaBalpha will occlude the NF-kappaB DNA-binding cleft. The orientation of IkappaBalpha in the complex places its N- and C-terminal regions in appropriate locations for their known regulatory functions. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NFI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1NFI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NFI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Harrison, S | + | [[Category: Harrison, S C.]] |
| - | [[Category: Jacobs, M | + | [[Category: Jacobs, M D.]] |
[[Category: ankyrin repeat]] | [[Category: ankyrin repeat]] | ||
[[Category: complex (transcription regulation/ank repeat)]] | [[Category: complex (transcription regulation/ank repeat)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:30 2008'' |
Revision as of 12:05, 21 February 2008
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I-KAPPA-B-ALPHA/NF-KAPPA-B COMPLEX
Contents |
Overview
The inhibitory protein, IkappaBalpha, sequesters the transcription factor, NF-kappaB, as an inactive complex in the cytoplasm. The structure of the IkappaBalpha ankyrin repeat domain, bound to a partially truncated NF-kappaB heterodimer (p50/ p65), has been determined by X-ray crystallography at 2.7 A resolution. It shows a stack of six IkappaBalpha ankyrin repeats facing the C-terminal domains of the NF-kappaB Rel homology regions. Contacts occur in discontinuous patches, suggesting a combinatorial quality for ankyrin repeat specificity. The first two repeats cover an alpha helically ordered segment containing the p65 nuclear localization signal. The position of the sixth ankyrin repeat shows that full-length IkappaBalpha will occlude the NF-kappaB DNA-binding cleft. The orientation of IkappaBalpha in the complex places its N- and C-terminal regions in appropriate locations for their known regulatory functions.
Disease
Known diseases associated with this structure: Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency OMIM:[164008]
About this Structure
1NFI is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of an IkappaBalpha/NF-kappaB complex., Jacobs MD, Harrison SC, Cell. 1998 Dec 11;95(6):749-58. PMID:9865693
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