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| | {{STRUCTURE_1n1k| PDB=1n1k | SCENE= }} | | {{STRUCTURE_1n1k| PDB=1n1k | SCENE= }} |
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| - | '''NMR Structure for d(CCGCGG)2'''
| + | ===NMR Structure for d(CCGCGG)2=== |
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| - | ==Overview==
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| - | Long repeated stretches of d(CCG) and tri-nucleotide are crucial mutations that cause hereditary forms of mental retardation (fragile X-syndrome). Moreover, the alternating (CG) di-nucleotide is one of the candidates for Z-DNA conformation. Solution NMR structure of d(CCGCGG)(2) has been solved and is discussed. The determined NMR solution structure is a distorted highly bent B-DNA conformation with increased flexibility in both terminal residues. This conformation differs significantly from the Z-DNA tetramer structure reported for the same hexamer in the crystal state at similar ionic strength by Malinina and co-workers. Crystal structure of d(CCGCGG)(2) at high salt concentration includes a central alternating tetramer in Z-DNA conformation, while the initial cytosine swings out and forms a Watson-Crick base-pair with the terminal guanine of a symmetry-related molecule. In solution, NMR data for sugar ring puckering combined with restrained molecular dynamics simulations starting from a Z-DNA form show that terminal furanose residues could adopt the conformation required for aromatic bases swinging out. Therefore, tetramer formation could be considered possible once the hexanucleotide had previously adopted the Z-DNA form. This work gives some insight into correlations between anomalous crystal structures and their accessibility in the solution state.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12646170}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12646170 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12646170}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1K OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1K OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Nmr]] | | [[Category: Nmr]] |
| | [[Category: Recombination-like]] | | [[Category: Recombination-like]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:58:34 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:17:41 2008'' |
Revision as of 01:17, 28 July 2008
Template:STRUCTURE 1n1k
NMR Structure for d(CCGCGG)2
Template:ABSTRACT PUBMED 12646170
About this Structure
Full experimental information is available from OCA.
Reference
NMR study of hexanucleotide d(CCGCGG)2 containing two triplet repeats of fragile X syndrome., Monleon D, Esteve V, Celda B, Biochem Biophys Res Commun. 2003 Mar 28;303(1):81-90. PMID:12646170
Page seeded by OCA on Mon Jul 28 04:17:41 2008
