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1nhl
From Proteopedia
(New page: 200px<br /> <applet load="1nhl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nhl, resolution 2.3Å" /> '''SNAP-23N Structure''...) |
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'''SNAP-23N Structure'''<br /> | '''SNAP-23N Structure'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NHL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1NHL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: snare]] | [[Category: snare]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:29:01 2008'' |
Revision as of 14:29, 15 February 2008
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SNAP-23N Structure
Overview
SNARE proteins mediate intracellular membrane fusion by forming a, coiled-coil complex to merge opposing membranes. A "fusion-active", neuronal SNARE complex is a parallel four-helix bundle containing two, coiled-coil domains from SNAP-25 and one coiled-coil domain each from, syntaxin-1a and VAMP-2. "Prefusion" assembly intermediate complexes can, also form from these SNAREs. We studied the N-terminal coiled-coil domain, of SNAP-23 (SNAP-23N), a non-neuronal homologue of SNAP-25, and its, interaction with other coiled-coil domains. SNAP-23N can assemble, spontaneously with the coiled-coil domains from SNAP-23C, syntaxin-4, and, VAMP-3 to form a heterotetrameric complex. Unexpectedly, pure SNAP-23N, crystallizes as a coiled-coil homotetrameric complex. The four helices, have a parallel orientation and are symmetrical about the long axis. The, complex is stabilized through the interaction of conserved hydrophobic, residues comprising the a and d positions of the coiled-coil heptad, repeats. In addition, a central, highly conserved glutamine residue, (Gln-48) is buried within the interface by hydrogen bonding between, glutamine side chains derived from adjacent subunits and to solvent, molecules. A comparison of the SNAP-23N structure to other SNARE complex, structures reveals how a simple coiled-coil motif can form diverse SNARE, complexes.
About this Structure
1NHL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain., Freedman SJ, Song HK, Xu Y, Sun ZY, Eck MJ, J Biol Chem. 2003 Apr 11;278(15):13462-7. Epub 2003 Jan 29. PMID:12556468
Page seeded by OCA on Fri Feb 15 16:29:01 2008
Categories: Homo sapiens | Single protein | Eck, M.J. | Freedman, S.J. | Song, H.K. | Xu, Y. | Coiled-coil | Snare
