1n48
From Proteopedia
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{{STRUCTURE_1n48| PDB=1n48 | SCENE= }} | {{STRUCTURE_1n48| PDB=1n48 | SCENE= }} | ||
| - | + | ===Y-family DNA polymerase Dpo4 in complex with DNA containing abasic lesion=== | |
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| - | ==Overview== | ||
| - | Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) is a DinB homolog that belongs to the recently described Y-family of DNA polymerases, which are best characterized by their low-fidelity synthesis on undamaged DNA templates and propensity to traverse normally replication-blocking lesions. Crystal structures of Dpo4 in ternary complexes with DNA and an incoming nucleotide, either correct or incorrect, have been solved at 1.7 A and 2.1 A resolution, respectively. Despite a conserved active site and a hand-like configuration similar to all known polymerases, Dpo4 makes limited and nonspecific contacts with the replicating base pair, thus relaxing base selection. Dpo4 is also captured in the crystal translocating two template bases to the active site at once, suggesting a possible mechanism for bypassing thymine dimers. | ||
==About this Structure== | ==About this Structure== | ||
| - | 1N48 is a | + | 1N48 is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N48 OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:11595188</ref><references group="xtra"/> | |
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Sulfolobus solfataricus]] | [[Category: Sulfolobus solfataricus]] | ||
[[Category: Boudsocq, F.]] | [[Category: Boudsocq, F.]] | ||
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[[Category: Protein-dna complex]] | [[Category: Protein-dna complex]] | ||
[[Category: Y-family]] | [[Category: Y-family]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 09:10:50 2009'' | ||
Revision as of 07:10, 17 February 2009
Y-family DNA polymerase Dpo4 in complex with DNA containing abasic lesion
About this Structure
1N48 is a 3 chains structure of sequences from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
- Ling H, Boudsocq F, Woodgate R, Yang W. Crystal structure of a Y-family DNA polymerase in action: a mechanism for error-prone and lesion-bypass replication. Cell. 2001 Oct 5;107(1):91-102. PMID:11595188
Page seeded by OCA on Tue Feb 17 09:10:50 2009
