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1n4f

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[[Image:1n4f.jpg|left|200px]]
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{{Seed}}
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{{STRUCTURE_1n4f| PDB=1n4f | SCENE= }}
{{STRUCTURE_1n4f| PDB=1n4f | SCENE= }}
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'''Para-Arsanilate Derivative of Hen Egg-White Lysozyme'''
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===Para-Arsanilate Derivative of Hen Egg-White Lysozyme===
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==Overview==
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Single/multiple-wavelength anomalous dispersion (SAD/MAD) experiments were performed on a crystal of an organic arsenic derivative of hen egg-white lysozyme. A para-arsanilate compound used as a crystallizing reagent was incorporated into the ordered solvent region of the lysozyme molecule. Diffraction data were collected to high resolution (&lt;/=2.0 A) at three wavelengths around the K edge (1.04 A) of arsenic at beamline BM30A, ESRF synchrotron. Anomalous Patterson maps clearly showed the main arsanilate site to be between three symmetry-related lysozyme molecules, at a location previously occupied by a para-toluenesulfonate anion. MAD phases at 2 A derived using the program SHARP led to an electron-density map of sufficient quality to start manual building of the protein model. Amplitudes from a second crystal measured to a resolution of 1.8 A at the peak wavelength revealed two additional heavy-atom sites, which reinforced the anomalous subset model and therefore dramatically improved the phasing power of the arsenic derivative. The subsequent solvent-flattened map was of such high accuracy that the program ARP/wARP was able to build a nearly complete model automatically. This work emphasizes the great potential of arsenic for de novo structure determination using anomalous dispersion methods.
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(as it appears on PubMed at http://www.pubmed.gov), where 12777806 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12777806}}
==About this Structure==
==About this Structure==
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[[Category: Retailleau, P.]]
[[Category: Retailleau, P.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:05:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:58:13 2008''

Revision as of 19:58, 27 July 2008

Image:1n4f.png

Template:STRUCTURE 1n4f

Para-Arsanilate Derivative of Hen Egg-White Lysozyme

Template:ABSTRACT PUBMED 12777806

About this Structure

1N4F is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Phasing power at the K absorption edge of organic arsenic., Retailleau P, Prange T, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):887-96. Epub 2003, Apr 25. PMID:12777806

Page seeded by OCA on Sun Jul 27 22:58:13 2008

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