We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1n5c

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1n5c.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1n5c.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1n5c| PDB=1n5c | SCENE= }}
{{STRUCTURE_1n5c| PDB=1n5c | SCENE= }}
-
'''Crystal Structure Analysis of the B-DNA Dodecamer CGCGAATT(ethenoC)GCG'''
+
===Crystal Structure Analysis of the B-DNA Dodecamer CGCGAATT(ethenoC)GCG===
-
==Overview==
+
<!--
-
Exocyclic DNA adducts are formed from metabolites of chemical carcinogens and have also been detected as endogenous lesions in human DNA. The exocyclic adduct 3,N(4)-etheno-2'-deoxycytidine (epsilon dC), positioned opposite deoxyguanosine in the B-form duplex of the dodecanucleotide d(CGCGAATTepsilonCGCG), has been crystallographically characterized at 1.8A resolution. This self-complementary oligomer crystallizes in space group P3(2)12, containing a single strand in the asymmetric unit. The crystal structure was solved by isomorphous replacement with the corresponding unmodified dodecamer structure. Exposure of both structures to identical crystal packing forces allows a detailed investigation of the influence of the exocyclic base adduct on the overall helical structure and local geometry. Structural changes are limited to the epsilon C:G and adjacent T:A and G:C base-pairs. The standard Watson-Crick base-pairing scheme, retained in the T:A and G:C base-pairs, is blocked by the etheno bridge in the epsilon C:G pair. In its place, a hydrogen bond involving O2 of epsilon C and N1 of G is present. Comparison with an epsilon dC-containing NMR structure confirms the general conformation reported for epsilon C:G, including the hydrogen bonding features. Superposition with the crystal structure of a DNA duplex containing a T:G wobble pair shows similar structural changes imposed by both mismatches. Evaluation of the hydration shell of the duplex with bond valence calculations reveals two sodium ions in the crystal.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12787670}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12787670 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12787670}}
==About this Structure==
==About this Structure==
Line 26: Line 30:
[[Category: 3,n4-etheno-2'-cytidine modification opposite g]]
[[Category: 3,n4-etheno-2'-cytidine modification opposite g]]
[[Category: B form double helix]]
[[Category: B form double helix]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:06:55 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 13 15:17:08 2008''

Revision as of 12:17, 13 July 2008

Image:1n5c.png

Template:STRUCTURE 1n5c

Crystal Structure Analysis of the B-DNA Dodecamer CGCGAATT(ethenoC)GCG

Template:ABSTRACT PUBMED 12787670

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystallographic characterization of an exocyclic DNA adduct: 3,N4-etheno-2'-deoxycytidine in the dodecamer 5'-CGCGAATTepsilonCGCG-3'., Freisinger E, Fernandes A, Grollman AP, Kisker C, J Mol Biol. 2003 Jun 13;329(4):685-97. PMID:12787670

Page seeded by OCA on Sun Jul 13 15:17:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n5c"
Personal tools