1n75

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{{STRUCTURE_1n75| PDB=1n75 | SCENE= }}
{{STRUCTURE_1n75| PDB=1n75 | SCENE= }}
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'''Crystal structure of Thermus thermophilus glutamyl-tRNA synthetase complexed with ATP.'''
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===Crystal structure of Thermus thermophilus glutamyl-tRNA synthetase complexed with ATP.===
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==Overview==
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Aminoacyl-tRNA synthetases catalyze the formation of an aminoacyl-AMP from an amino acid and ATP, prior to the aminoacyl transfer to tRNA. A subset of aminoacyl-tRNA synthetases, including glutamyl-tRNA synthetase (GluRS), have a regulation mechanism to avoid aminoacyl-AMP formation in the absence of tRNA. In this study, we determined the crystal structure of the 'non-productive' complex of Thermus thermophilus GluRS, ATP and L-glutamate, together with those of the GluRS.ATP, GluRS.tRNA.ATP and GluRS.tRNA.GoA (a glutamyl-AMP analog) complexes. In the absence of tRNA(Glu), ATP is accommodated in a 'non-productive' subsite within the ATP-binding site, so that the ATP alpha-phosphate and the glutamate alpha-carboxyl groups in GluRS. ATP.Glu are too far from each other (6.2 A) to react. In contrast, the ATP-binding mode in GluRS.tRNA. ATP is dramatically different from those in GluRS.ATP.Glu and GluRS.ATP, but corresponds to the AMP moiety binding mode in GluRS.tRNA.GoA (the 'productive' subsite). Therefore, tRNA binding to GluRS switches the ATP-binding mode. The interactions of the three tRNA(Glu) regions with GluRS cause conformational changes around the ATP-binding site, and allow ATP to bind to the 'productive' subsite.
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(as it appears on PubMed at http://www.pubmed.gov), where 12554668 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12554668}}
==About this Structure==
==About this Structure==
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:10:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:33:53 2008''

Revision as of 07:33, 28 July 2008

Image:1n75.png

Template:STRUCTURE 1n75

Crystal structure of Thermus thermophilus glutamyl-tRNA synthetase complexed with ATP.

Template:ABSTRACT PUBMED 12554668

About this Structure

1N75 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

ATP binding by glutamyl-tRNA synthetase is switched to the productive mode by tRNA binding., Sekine S, Nureki O, Dubois DY, Bernier S, Chenevert R, Lapointe J, Vassylyev DG, Yokoyama S, EMBO J. 2003 Feb 3;22(3):676-88. PMID:12554668

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