1n7f

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{{STRUCTURE_1n7f| PDB=1n7f | SCENE= }}
{{STRUCTURE_1n7f| PDB=1n7f | SCENE= }}
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'''Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide'''
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===Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide===
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==Overview==
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PDZ domains bind to short segments within target proteins in a sequence-specific fashion. Glutamate receptor-interacting protein (GRIP)/ABP family proteins contain six to seven PDZ domains and interact via the sixth PDZ domain (class II) with the C termini of various proteins including liprin-alpha. In addition the PDZ456 domain mediates the formation of homo- and heteromultimers of GRIP proteins. To better understand the structural basis of peptide recognition by a class II PDZ domain and PDZ-mediated multimerization, we determined the crystal structures of the GRIP1 PDZ6 domain alone and in complex with a synthetic C-terminal octapeptide of human liprin-alpha at resolutions of 1.5 and 1.8 A, respectively. Remarkably, unlike other class II PDZ domains, Ile-736 at alphaB5 rather than conserved Leu-732 at alphaB1 makes a direct hydrophobic contact with the side chain of the Tyr at the -2 position of the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight reorientation of helix alphaB, indicating that the second hydrophobic pocket undergoes a conformational adaptation to accommodate the bulkiness of the Tyr side chain, and forms an antiparallel dimer through an interface located at a site distal to the peptide-binding groove. This configuration may enable formation of GRIP multimers and efficient clustering of GRIP-binding proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 12493751 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12493751}}
==About this Structure==
==About this Structure==
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[[Category: Liprin]]
[[Category: Liprin]]
[[Category: Pdz]]
[[Category: Pdz]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:39:06 2008''

Revision as of 00:39, 29 July 2008

Image:1n7f.png

Template:STRUCTURE 1n7f

Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide

Template:ABSTRACT PUBMED 12493751

About this Structure

1N7F is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Crystal structure of GRIP1 PDZ6-peptide complex reveals the structural basis for class II PDZ target recognition and PDZ domain-mediated multimerization., Im YJ, Park SH, Rho SH, Lee JH, Kang GB, Sheng M, Kim E, Eom SH, J Biol Chem. 2003 Mar 7;278(10):8501-7. Epub 2002 Dec 18. PMID:12493751

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