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1n87

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[[Image:1n87.gif|left|200px]]
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{{STRUCTURE_1n87| PDB=1n87 | SCENE= }}
{{STRUCTURE_1n87| PDB=1n87 | SCENE= }}
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'''Solution structure of the U-box of Prp19'''
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===Solution structure of the U-box of Prp19===
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==Overview==
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The structure of the U-box in the essential Saccharomyces cerevisiae pre-mRNA splicing factor Prp19p has been determined by NMR. The conserved zinc-binding sites supporting the cross-brace arrangement in RING-finger domains are replaced by hydrogen-bonding networks in the U-box. These hydrogen-bonding networks are necessary for the structural stabilization and activity of the U-box. A conservative Val--&gt;Ile point mutation in the Prp19p U-box domain leads to pre-mRNA splicing defects in vivo. NMR analysis of this mutant shows that the substitution disrupts structural integrity of the U-box domain. Furthermore, comparison of the Prp19p U-box domain with known RING-E2 complex structures demonstrates that both U-box and RING-fingers contain a conserved interaction surface. Mutagenesis of residues at this interface, while not perturbing the structure of the U-box, abrogates Prp19p function in vivo. These comparative structural and functional analyses imply that the U-box and its associated ubiquitin ligase activity are critical for Prp19p function in vivo.
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The line below this paragraph, {{ABSTRACT_PUBMED_12627222}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12627222 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12627222}}
==About this Structure==
==About this Structure==
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1N87 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N87 OCA].
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1N87 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N87 OCA].
==Reference==
==Reference==
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[[Category: U-box]]
[[Category: U-box]]
[[Category: Ubiquitin ligase]]
[[Category: Ubiquitin ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:13:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:39:00 2008''

Revision as of 19:39, 28 July 2008

Image:1n87.png

Template:STRUCTURE 1n87

Solution structure of the U-box of Prp19

Template:ABSTRACT PUBMED 12627222

About this Structure

1N87 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

Reference

Structural insights into the U-box, a domain associated with multi-ubiquitination., Ohi MD, Vander Kooi CW, Rosenberg JA, Chazin WJ, Gould KL, Nat Struct Biol. 2003 Apr;10(4):250-5. PMID:12627222

Page seeded by OCA on Mon Jul 28 22:39:00 2008

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