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1n8m

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{{STRUCTURE_1n8m| PDB=1n8m | SCENE= }}
{{STRUCTURE_1n8m| PDB=1n8m | SCENE= }}
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'''Solution structure of Pi4, a four disulfide bridged scorpion toxin active on potassium channels'''
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===Solution structure of Pi4, a four disulfide bridged scorpion toxin active on potassium channels===
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==Overview==
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Pi4 is a short toxin found at very low abundance in the venom of Pandinus imperator scorpions. It is a potent blocker of K(+) channels. Like the other members of the alpha-KTX6 subfamily to which it belongs, it is cross-linked by four disulfide bonds. The synthetic analog (sPi4) and the natural toxin (nPi4) have been obtained by solid-phase synthesis or from scorpion venom, respectively. Analysis of two-dimensional (1)H NMR spectra of nPi4 and sPi4 indicates that both peptides have the same structure. Moreover, electrophysiological recordings of the blocking of Shaker B K(+) channels by sPi4 (K(D) = 8.5 nM) indicate that sPi4 has the same blocking activity of nPi4 (K(D) = 8.0 nM), previously described. The disulfide bonds have been independently determined by NMR and structure calculations, and by Edman-degradation/mass-spectrometry identification of peptides obtained by proteolysis of nPi4. Both approaches indicate that the pairing of the half-cystines is (6)C-(27)C, (12)C-(32)C, (16)C-(34)C, and (22)C-(37)C. The structure of the toxin has been determined by using 705 constraints derived from NMR data on sPi4. The structure, which is well defined, shows the characteristic alpha/beta scaffold of scorpion toxins. It is compared to the structure of the other alpha-KTX6 subfamily members and, in particular, to the structure of maurotoxin, which shows a different pattern of disulfide bridges despite its high degree of sequence identity (76%) with Pi4. The structure of Pi4 and the high amounts of synthetic peptide available, will enable the detailed analysis of the interaction of Pi4 with K(+) channels.
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(as it appears on PubMed at http://www.pubmed.gov), where 12930984 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12930984}}
==About this Structure==
==About this Structure==
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1N8M is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8M OCA].
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1N8M is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8M OCA].
==Reference==
==Reference==
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[[Category: Disulfide bridge stabilized alpha beta motif]]
[[Category: Disulfide bridge stabilized alpha beta motif]]
[[Category: Potassium channel blocker]]
[[Category: Potassium channel blocker]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:13:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:19:02 2008''

Revision as of 08:19, 29 July 2008

Image:1n8m.png

Template:STRUCTURE 1n8m

Solution structure of Pi4, a four disulfide bridged scorpion toxin active on potassium channels

Template:ABSTRACT PUBMED 12930984

About this Structure

1N8M is a Single protein structure. Full experimental information is available from OCA.

Reference

Solution structure of Pi4, a short four-disulfide-bridged scorpion toxin specific of potassium channels., Guijarro JI, M'Barek S, Gomez-Lagunas F, Garnier D, Rochat H, Sabatier JM, Possani L, Delepierre M, Protein Sci. 2003 Sep;12(9):1844-54. PMID:12930984

Page seeded by OCA on Tue Jul 29 11:19:02 2008

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