1nrg
From Proteopedia
(New page: 200px<br /> <applet load="1nrg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nrg, resolution 1.95Å" /> '''Structure and Prope...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1nrg. | + | [[Image:1nrg.jpg|left|200px]]<br /><applet load="1nrg" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1nrg" size=" | + | |
caption="1nrg, resolution 1.95Å" /> | caption="1nrg, resolution 1.95Å" /> | ||
'''Structure and Properties of Recombinant Human Pyridoxine-5'-Phosphate Oxidase'''<br /> | '''Structure and Properties of Recombinant Human Pyridoxine-5'-Phosphate Oxidase'''<br /> | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1NRG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with PO4, FMN, PLP and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1NRG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=FMN:'>FMN</scene>, <scene name='pdbligand=PLP:'>PLP</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NRG OCA]. |
==Reference== | ==Reference== | ||
| Line 31: | Line 30: | ||
[[Category: pyridoxine-5'-phosphate]] | [[Category: pyridoxine-5'-phosphate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:30:37 2008'' |
Revision as of 14:30, 15 February 2008
|
Structure and Properties of Recombinant Human Pyridoxine-5'-Phosphate Oxidase
Contents |
Overview
Pyridoxine 5'-phosphate oxidase catalyzes the terminal step in the, synthesis of pyridoxal 5'-phosphate. The cDNA for the human enzyme has, been cloned and expressed in Escherichia coli. The purified human enzyme, is a homodimer that exhibits a low catalytic rate constant of, approximately 0.2 sec(-1) and K(m) values in the low micromolar range for, both pyridoxine 5'phosphate and pyridoxamine 5'-phosphate. Pyridoxal, 5'-phosphate is an effective product inhibitor. The three-dimensional fold, of the human enzyme is very similar to those of the E. coli and yeast, enzymes. The human and E. coli enzymes share 39% sequence identity, but, the binding sites for the tightly bound FMN and substrate are highly, conserved. As observed with the E. coli enzyme, the human enzyme binds one, molecule of pyridoxal 5'-phosphate tightly on each subunit.
Disease
Known disease associated with this structure: Pyridoxamine 5 -phosphate oxidase deficiency OMIM:[603287]
About this Structure
1NRG is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase., Musayev FN, Di Salvo ML, Ko TP, Schirch V, Safo MK, Protein Sci. 2003 Jul;12(7):1455-63. PMID:12824491
Page seeded by OCA on Fri Feb 15 16:30:37 2008
Categories: Homo sapiens | Single protein | Ko, T.P. | Musayev, F.N. | Safo, M.K. | Salvo, M.L.di. | Schirch, V. | BME | FMN | PLP | PO4 | Fmn | Oxidase | Plp | Pyridoxine-5'-phosphate
