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| | {{STRUCTURE_1nap| PDB=1nap | SCENE= }} | | {{STRUCTURE_1nap| PDB=1nap | SCENE= }} |
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| - | '''THE CRYSTAL STRUCTURE OF RECOMBINANT HUMAN NEUTROPHIL-ACTIVATING PEPTIDE-2 (M6L) AT 1.9-ANGSTROMS RESOLUTION'''
| + | ===THE CRYSTAL STRUCTURE OF RECOMBINANT HUMAN NEUTROPHIL-ACTIVATING PEPTIDE-2 (M6L) AT 1.9-ANGSTROMS RESOLUTION=== |
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| - | ==Overview==
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| - | Neutrophil-activating peptide-2 (NAP-2) is a 70-residue carboxyl-terminal fragment of platelet basic protein, which is found in the alpha-granules of human platelets. NAP-2, which belongs to the CXC family of chemokines that includes interleukin-8 and platelet factor 4, binds to the interleukin-8 type II receptor and induces a rise in cytosolic calcium, chemotaxis of neutrophils, and exocytosis. Crystals of recombinant NAP-2 in which the single methionine at position 6 was replaced by leucine to facilitate expression belong to space group P1 (unit cell parameters a = 40.8, b = 43.8, and c = 44.7 A and alpha = 98.4 degrees, beta = 120.3 degrees, and gamma = 92.8 degrees), with 4 molecules of NAP-2 (Mr = 7600) in the asymmetric unit. The molecular replacement solution calculated with bovine platelet factor 4 as the starting model was refined using rigid body refinement, manual fitting in solvent-leveled electron density maps, simulated annealing, and restrained least squares to an R-factor of 0.188 for 2 sigma data between 7.0- and 1.9-A resolution. The final refined crystal structure includes 265 solvent molecules. The overall tertiary structure, which is similar to that of platelet factor 4 and interleukin-8, includes an extended amino-terminal loop, three strands of antiparallel beta-sheet arranged in a Greek key fold, and one alpha-helix at the carboxyl terminus. The Glu-Leu-Arg sequence that is critical for receptor binding is fully defined by electron density and exhibits multiple conformations.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_7706245}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 7706245 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_7706245}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Malkowski, M G.]] | | [[Category: Malkowski, M G.]] |
| | [[Category: Cytokine]] | | [[Category: Cytokine]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:18:06 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:38:02 2008'' |
Revision as of 04:38, 28 July 2008
Template:STRUCTURE 1nap
THE CRYSTAL STRUCTURE OF RECOMBINANT HUMAN NEUTROPHIL-ACTIVATING PEPTIDE-2 (M6L) AT 1.9-ANGSTROMS RESOLUTION
Template:ABSTRACT PUBMED 7706245
About this Structure
1NAP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystal structure of recombinant human neutrophil-activating peptide-2 (M6L) at 1.9-A resolution., Malkowski MG, Wu JY, Lazar JB, Johnson PH, Edwards BF, J Biol Chem. 1995 Mar 31;270(13):7077-87. PMID:7706245
Page seeded by OCA on Mon Jul 28 07:38:02 2008
