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| | {{STRUCTURE_1nbr| PDB=1nbr | SCENE= }} | | {{STRUCTURE_1nbr| PDB=1nbr | SCENE= }} |
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| - | '''Iron Responsive Element RNA Hairpin, NMR, 15 Structures'''
| + | ===Iron Responsive Element RNA Hairpin, NMR, 15 Structures=== |
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| - | ==Overview==
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| - | The iron-responsive element (IRE) is a 30nt RNA motif located in the non-coding regions of mRNAs of proteins involved in iron regulation. In humans, the IRE plays a direct role in the control of iron levels by post-transcriptional regulation of the ferritin and transferrin receptor proteins through highly specific recognition by IRE-binding proteins. The IRE fold is representative of many RNA motifs that contain helical domains separated by a bulge or internal loop. The global structures of such extended multi-domain RNAs are not well defined by conventional NMR-distance and torsion angle structural restraints. Residual dipolar couplings (RDCs) are employed here to better define the global structure of the IRE RNA in solution. RDCs contain valuable long-range structural information that compliments the short-range structural data derived from standard NOE-distance and torsion angle restraints. Several approaches for estimating alignment tensor parameters and incorporating RDCs into RNA structure determinations are compared. Both the local and global structure of the IRE are improved significantly by refinement with RDCs. These RDC refinements provide insight on the conformational dynamics of the IRE. These studies highlight some issues that need to be addressed when incorporating RDCs in solution structure determinations of nucleic acids. The approach used here should prove valuable for structure determinations of various multi-domain systems. | + | The line below this paragraph, {{ABSTRACT_PUBMED_12589752}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12589752 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12589752}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBR OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBR OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Rna]] | | [[Category: Rna]] |
| | [[Category: Translational regulation]] | | [[Category: Translational regulation]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:20:34 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:00:31 2008'' |
Revision as of 10:00, 29 July 2008
Template:STRUCTURE 1nbr
Iron Responsive Element RNA Hairpin, NMR, 15 Structures
Template:ABSTRACT PUBMED 12589752
About this Structure
Full experimental information is available from OCA.
Reference
Refined solution structure of the iron-responsive element RNA using residual dipolar couplings., McCallum SA, Pardi A, J Mol Biol. 2003 Feb 28;326(4):1037-50. PMID:12589752
Page seeded by OCA on Tue Jul 29 13:00:31 2008
