1nvp
From Proteopedia
(New page: 200px<br /> <applet load="1nvp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nvp, resolution 2.10Å" /> '''HUMAN TFIIA/TBP/DNA...) |
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| - | [[Image:1nvp.gif|left|200px]]<br /> | + | [[Image:1nvp.gif|left|200px]]<br /><applet load="1nvp" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1nvp" size=" | + | |
caption="1nvp, resolution 2.10Å" /> | caption="1nvp, resolution 2.10Å" /> | ||
'''HUMAN TFIIA/TBP/DNA COMPLEX'''<br /> | '''HUMAN TFIIA/TBP/DNA COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | RNA polymerase II-dependent transcription requires the assembly of a | + | RNA polymerase II-dependent transcription requires the assembly of a multi-protein, preinitiation complex on core promoter elements. Transcription factor IID (TFIID) comprising the TATA box-binding protein (TBP) and TBP-associated factors (TAFs) is responsible for promoter recognition in this complex. Subsequent association of TFIIA and TFIIB provides enhanced complex stability. TFIIA is required for transcriptional stimulation by certain viral and cellular activators, and favors formation of the preinitiation complex in the presence of repressor NC2. The X-ray structures of human and yeast TBP/TFIIA/DNA complexes at 2.1A and 1.9A resolution, respectively, are presented here and seen to resemble each other closely. The interactions made by human TFIIA with TBP and DNA within and upstream of the TATA box, including those involving water molecules, are described and compared to the yeast structure. Of particular interest is a previously unobserved region of TFIIA that extends the binding interface with TBP in the yeast, but not in the human complex, and that further elucidates biochemical and genetic results. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NVP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1NVP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NVP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bleichenbacher, M.]] | [[Category: Bleichenbacher, M.]] | ||
| - | [[Category: Richmond, T | + | [[Category: Richmond, T J.]] |
[[Category: Tan, S.]] | [[Category: Tan, S.]] | ||
[[Category: complex]] | [[Category: complex]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:10:33 2008'' |
Revision as of 12:10, 21 February 2008
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HUMAN TFIIA/TBP/DNA COMPLEX
Contents |
Overview
RNA polymerase II-dependent transcription requires the assembly of a multi-protein, preinitiation complex on core promoter elements. Transcription factor IID (TFIID) comprising the TATA box-binding protein (TBP) and TBP-associated factors (TAFs) is responsible for promoter recognition in this complex. Subsequent association of TFIIA and TFIIB provides enhanced complex stability. TFIIA is required for transcriptional stimulation by certain viral and cellular activators, and favors formation of the preinitiation complex in the presence of repressor NC2. The X-ray structures of human and yeast TBP/TFIIA/DNA complexes at 2.1A and 1.9A resolution, respectively, are presented here and seen to resemble each other closely. The interactions made by human TFIIA with TBP and DNA within and upstream of the TATA box, including those involving water molecules, are described and compared to the yeast structure. Of particular interest is a previously unobserved region of TFIIA that extends the binding interface with TBP in the yeast, but not in the human complex, and that further elucidates biochemical and genetic results.
Disease
Known diseases associated with this structure: Huntington disease-like-4 OMIM:[600075], Parkinson disease OMIM:[600075], Spinocerebellar ataxia 17 OMIM:[600075]
About this Structure
1NVP is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes., Bleichenbacher M, Tan S, Richmond TJ, J Mol Biol. 2003 Sep 26;332(4):783-93. PMID:12972251
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