1nfh

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{{STRUCTURE_1nfh| PDB=1nfh | SCENE= }}
{{STRUCTURE_1nfh| PDB=1nfh | SCENE= }}
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'''Structure of a Sir2 substrate, alba, reveals a mechanism for deactylation-induced enhancement of DNA-binding'''
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===Structure of a Sir2 substrate, alba, reveals a mechanism for deactylation-induced enhancement of DNA-binding===
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==Overview==
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The targeted acetylation status of histones and several other transcriptional regulatory proteins plays an important role in gene expression, although the mechanism for this is not well understood. As a model to understand how targeted acetylation may effect transcription, we determined the x-ray crystal structure of the chromatin protein Alba from Archaeoglobus fulgidus, a substrate for the Sir2 protein that deacetylates it at lysine 11 to promote DNA binding by Alba. The structure reveals a dimer of dimers in which the dimer-dimer interface is stabilized by several conserved hydrophobic residues as well as the lysine 11 target of Sir2. We show that, in solution, the mutation of these hydrophobic residues or lysine 11 disrupts dimer-dimer formation and decreases DNA-binding affinity. We propose that the in vivo deacetylation of lysine 11 of archaeal Alba by Sir2 promotes protein oligomerization for optimal DNA binding. Implications for the mechanism by which histone acetylation modulates gene expression are discussed.
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{{ABSTRACT_PUBMED_12730210}}
==About this Structure==
==About this Structure==
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[[Category: Sir2]]
[[Category: Sir2]]
[[Category: Transcription]]
[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:28:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:53:39 2008''

Revision as of 02:53, 29 July 2008

Image:1nfh.png

Template:STRUCTURE 1nfh

Structure of a Sir2 substrate, alba, reveals a mechanism for deactylation-induced enhancement of DNA-binding

Template:ABSTRACT PUBMED 12730210

About this Structure

1NFH is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Structure of a Sir2 substrate, Alba, reveals a mechanism for deacetylation-induced enhancement of DNA binding., Zhao K, Chai X, Marmorstein R, J Biol Chem. 2003 Jul 11;278(28):26071-7. Epub 2003 May 1. PMID:12730210

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