1nft

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{{STRUCTURE_1nft| PDB=1nft | SCENE= }}
{{STRUCTURE_1nft| PDB=1nft | SCENE= }}
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'''OVOTRANSFERRIN, N-TERMINAL LOBE, IRON LOADED OPEN FORM'''
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===OVOTRANSFERRIN, N-TERMINAL LOBE, IRON LOADED OPEN FORM===
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==Overview==
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Transferrins bind Fe3+ very tightly in a closed interdomain cleft by the coordination of four protein ligands (Asp60, Tyr92, Tyr191, and His250 in ovotransferrin N-lobe) and of a synergistic anion, physiologically bidentate CO32-. Upon Fe3+ uptake, transferrins undergo a large scale conformational transition: the apo structure with an opening of the interdomain cleft is transformed into the closed holo structure, implying initial Fe3+ binding in the open form. To solve the Fe3+-loaded, domain-opened structure, an ovotransferrin N-lobe crystal that had been grown as the apo form was soaked with Fe3+-nitrilotriacetate, and its structure was solved at 2.1 A resolution. The Fe3+-soaked form showed almost exactly the same overall open structure as the iron-free apo form. The electron density map unequivocally proved the presence of an iron atom with the coordination by the two protein ligands of Tyr92-OH and Tyr191-OH. Other Fe3+ coordination sites are occupied by a nitrilotriacetate anion, which is stabilized through the hydrogen bonds with the peptide NH groups of Ser122, Ala123, and Gly124 and a side chain group of Thr117. There is, however, no clear interaction between the nitrilotriacetate anion and the synergistic anion binding site, Arg121.
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(as it appears on PubMed at http://www.pubmed.gov), where 10187803 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10187803}}
==About this Structure==
==About this Structure==
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[[Category: Ovotransferrin]]
[[Category: Ovotransferrin]]
[[Category: Transferrin]]
[[Category: Transferrin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:28:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:26:15 2008''

Revision as of 12:26, 27 July 2008

Image:1nft.png

Template:STRUCTURE 1nft

OVOTRANSFERRIN, N-TERMINAL LOBE, IRON LOADED OPEN FORM

Template:ABSTRACT PUBMED 10187803

About this Structure

1NFT is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Alternative structural state of transferrin. The crystallographic analysis of iron-loaded but domain-opened ovotransferrin N-lobe., Mizutani K, Yamashita H, Kurokawa H, Mikami B, Hirose M, J Biol Chem. 1999 Apr 9;274(15):10190-4. PMID:10187803

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