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1nj8

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[[Image:1nj8.gif|left|200px]]
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{{STRUCTURE_1nj8| PDB=1nj8 | SCENE= }}
{{STRUCTURE_1nj8| PDB=1nj8 | SCENE= }}
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'''Crystal Structure of Prolyl-tRNA Synthetase from Methanocaldococcus janaschii'''
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===Crystal Structure of Prolyl-tRNA Synthetase from Methanocaldococcus janaschii===
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==Overview==
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Cysteinyl-tRNA synthetase is an essential enzyme required for protein synthesis. Genes encoding this protein have not been identified in Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus, or Methanopyrus kandleri. It has previously been proposed that the prolyl-tRNA synthetase (ProRS) enzymes in these organisms recognize either proline or cysteine and can aminoacylate their cognate tRNAs through a dual-specificity mechanism. We report five crystal structures at resolutions between 2.6 and 3.2 A: apo M. jannaschii ProRS, and M. thermautotrophicus ProRS in apo form and in complex with cysteinyl-sulfamoyl-, prolyl-sulfamoyl-, and alanyl-sulfamoyl-adenylates. These aminoacyl-adenylate analogues bind to a single active-site pocket and induce an identical set of conformational changes in loops around the active site when compared with the ligand-free conformation of ProRS. The cysteinyl- and prolyl-adenylate analogues have similar, nanomolar affinities for M. thermautotrophicus ProRS. Homology modeling of tRNA onto these adenylate complexes places the 3'-OH of A76 in an appropriate position for the transfer of any of the three amino acids to tRNA. Thus, these structures explain recent biochemical experiments showing that M. jannaschii ProRS misacylates tRNA(Pro) with cysteine, and argue against the proposal that these archaeal ProRS enzymes possess the dual capacity to aminoacylate both tRNA(Pro) and tRNA(Cys) with their cognate amino acids.
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(as it appears on PubMed at http://www.pubmed.gov), where 12578991 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12578991}}
==About this Structure==
==About this Structure==
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[[Category: Wang, J.]]
[[Category: Wang, J.]]
[[Category: Class-ii trna synthetase]]
[[Category: Class-ii trna synthetase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:47:19 2008''

Revision as of 20:47, 27 July 2008

Image:1nj8.png

Template:STRUCTURE 1nj8

Crystal Structure of Prolyl-tRNA Synthetase from Methanocaldococcus janaschii

Template:ABSTRACT PUBMED 12578991

About this Structure

1NJ8 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

Reference

The structural basis of cysteine aminoacylation of tRNAPro by prolyl-tRNA synthetases., Kamtekar S, Kennedy WD, Wang J, Stathopoulos C, Soll D, Steitz TA, Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1673-8. Epub 2003 Feb 10. PMID:12578991

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