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1nk2

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[[Image:1nk2.gif|left|200px]]
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[[Image:1nk2.png|left|200px]]
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{{STRUCTURE_1nk2| PDB=1nk2 | SCENE= }}
{{STRUCTURE_1nk2| PDB=1nk2 | SCENE= }}
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'''VND/NK-2 HOMEODOMAIN/DNA COMPLEX, NMR, 20 STRUCTURES'''
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===VND/NK-2 HOMEODOMAIN/DNA COMPLEX, NMR, 20 STRUCTURES===
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==Overview==
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The interactions responsible for the nucleotide sequence-specific binding of the vnd/NK-2 homeodomain of Drosophila melanogaster to its consensus DNA binding site have been identified. A three-dimensional structure of the vnd/NK-2 homeodomain-DNA complex is presented, with emphasis on the structure of regions of observed protein-DNA contacts. This structure is based on protein-DNA distance restraints derived from NMR data, along with homology modeling, solvated molecular dynamics, and results from methylation and ethylation interference experiments. Helix III of the homeodomain binds in the major groove of the DNA and the N-terminal arm binds in the minor groove, in analogy with other homeodomain-DNA complexes whose structures have been reported. The vnd/NK-2 homeodomain recognizes the unusual DNA consensus sequence 5'-CAAGTG-3'. The roles in sequence specificity and strength of binding of individual amino acid residues that make contact with the DNA are described. We show, based primarily on the observed protein-DNA contacts, that the interaction of Y54 with the DNA is the major determinant of this uncommon nucleotide binding specificity in the vnd/NK-2 homeodomain-DNA complex.
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(as it appears on PubMed at http://www.pubmed.gov), where 9154919 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9154919}}
==About this Structure==
==About this Structure==
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1NK2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NK2 OCA].
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1NK2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NK2 OCA].
==Reference==
==Reference==
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[[Category: Homeobox]]
[[Category: Homeobox]]
[[Category: Homeodomain]]
[[Category: Homeodomain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:37:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:57:26 2008''

Revision as of 23:57, 27 July 2008

Image:1nk2.png

Template:STRUCTURE 1nk2

VND/NK-2 HOMEODOMAIN/DNA COMPLEX, NMR, 20 STRUCTURES

Template:ABSTRACT PUBMED 9154919

About this Structure

1NK2 is a Single protein structure of sequence from Drosophila melanogaster. Full experimental information is available from OCA.

Reference

Interactions of the vnd/NK-2 homeodomain with DNA by nuclear magnetic resonance spectroscopy: basis of binding specificity., Gruschus JM, Tsao DH, Wang LH, Nirenberg M, Ferretti JA, Biochemistry. 1997 May 6;36(18):5372-80. PMID:9154919

Page seeded by OCA on Mon Jul 28 02:57:26 2008

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