1nla

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{{STRUCTURE_1nla| PDB=1nla | SCENE= }}
{{STRUCTURE_1nla| PDB=1nla | SCENE= }}
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'''Solution Structure of Switch Arc, a Mutant with 3(10) Helices Replacing a Wild-Type Beta-Ribbon'''
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===Solution Structure of Switch Arc, a Mutant with 3(10) Helices Replacing a Wild-Type Beta-Ribbon===
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==Overview==
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Adjacent N11L and L12N mutations in the antiparallel beta-ribbon of Arc repressor result in dramatic changes in local structure in which each beta-strand is replaced by a right-handed helix. The full solution structure of this "switch" Arc mutant shows that irregular 3(10) helices compose the new secondary structure. This structural metamorphosis conserves the number of main-chain and side-chain to main-chain hydrogen bonds and the number of fully buried core residues. Apart from a slight widening of the interhelical angle between alpha-helices A and B and changes in side-chain conformation of a few core residues in Arc, no large-scale structural adjustments in the remainder of the protein are necessary to accommodate the ribbon-to-helix change. Nevertheless, some changes in hydrogen-exchange rates are observed, even in regions that have very similar structures in the two proteins. The surface of switch Arc is packed poorly compared to wild-type, leading to approximately 1000A(2) of additional solvent-accessible surface area, and the N termini of the 3(10) helices make unfavorable head-to-head electrostatic interactions. These structural features account for the positive m value and salt dependence of the ribbon-to-helix transition in Arc-N11L, a variant that can adopt either the mutant or wild-type structures. The tertiary fold is capped in different ways in switch and wild-type Arc, showing how stepwise evolutionary transformations can arise through small changes in amino acid sequence.
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(as it appears on PubMed at http://www.pubmed.gov), where 12581649 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12581649}}
==About this Structure==
==About this Structure==
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1NLA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NLA OCA].
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1NLA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NLA OCA].
==Reference==
==Reference==
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[[Category: Beta-sheet]]
[[Category: Beta-sheet]]
[[Category: Structural switching]]
[[Category: Structural switching]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:40:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:24:36 2008''

Revision as of 22:24, 27 July 2008

Image:1nla.png

Template:STRUCTURE 1nla

Solution Structure of Switch Arc, a Mutant with 3(10) Helices Replacing a Wild-Type Beta-Ribbon

Template:ABSTRACT PUBMED 12581649

About this Structure

1NLA is a Single protein structure of sequence from Enterobacteria phage p22. Full experimental information is available from OCA.

Reference

Solution structure of switch Arc, a mutant with 3(10) helices replacing a wild-type beta-ribbon., Cordes MH, Walsh NP, McKnight CJ, Sauer RT, J Mol Biol. 2003 Feb 21;326(3):899-909. PMID:12581649

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