1nnf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1nnf.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1nnf.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1nnf| PDB=1nnf | SCENE= }}
{{STRUCTURE_1nnf| PDB=1nnf | SCENE= }}
-
'''Crystal Structure Analysis of Haemophlius Influenzae Ferric-ion Binding Protein H9Q Mutant Form'''
+
===Crystal Structure Analysis of Haemophlius Influenzae Ferric-ion Binding Protein H9Q Mutant Form===
-
==Overview==
+
<!--
-
The periplasmic iron binding protein of pathogenic Gram-negative bacteria performs an essential role in iron acquisition from transferrin and other iron sources. Structural analysis of this protein from Haemophilus influenzae identified four amino acids that ligand the bound iron: His(9), Glu(57), Tyr(195), and Tyr(196). A phosphate provides an additional ligand, and the presence of a water molecule is required to complete the octahedral geometry for stable iron binding. We report the 1.14-A resolution crystal structure of the iron-loaded form of the H. influenzae periplasmic ferric ion binding protein (FbpA) mutant H9Q. This protein was produced in the periplasm of Escherichia coli and, after purification and conversion to the apo form, was iron-loaded. H9Q is able to bind ferric iron in an open conformation. A surprising finding in the present high resolution structure is the presence of EDTA located at the previously determined anion ternary binding site, where phosphate is located in the wild type holo and apo structures. EDTA contributes four of the six coordinating ligands for iron, with two Tyr residues, 195 and 196, completing the coordination. This is the first example of a metal binding protein with a bound metal.EDTA complex. The results suggest that FbpA may have the ability to bind and transport iron bound to biological chelators, in addition to bare ferric iron.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12533539}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12533539 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12533539}}
==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Edta-fe-protein complex]]
[[Category: Edta-fe-protein complex]]
[[Category: Iron-binding protein]]
[[Category: Iron-binding protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:44:53 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:18:08 2008''

Revision as of 17:18, 27 July 2008

Image:1nnf.png

Template:STRUCTURE 1nnf

Crystal Structure Analysis of Haemophlius Influenzae Ferric-ion Binding Protein H9Q Mutant Form

Template:ABSTRACT PUBMED 12533539

About this Structure

1NNF is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.

Reference

High resolution structure of an alternate form of the ferric ion binding protein from Haemophilus influenzae., Shouldice SR, Dougan DR, Skene RJ, Tari LW, McRee DE, Yu RH, Schryvers AB, J Biol Chem. 2003 Mar 28;278(13):11513-9. Epub 2003 Jan 17. PMID:12533539

Page seeded by OCA on Sun Jul 27 20:18:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nnf"
Personal tools