1np3

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{{STRUCTURE_1np3| PDB=1np3 | SCENE= }}
{{STRUCTURE_1np3| PDB=1np3 | SCENE= }}
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'''Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa'''
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===Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa===
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==Overview==
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Acetohydroxy acid isomeroreductase (AHIR) is a key enzyme in the biosynthesis of branched-chain amino acids. We have determined the first crystal structure of a class I AHIR from Pseudomonas aeruginosa at 2.0 A resolution. Its dodecameric architecture of 23 point group symmetry is assembled of six dimeric units and dimerization is essential for the formation of the active site. The dimeric unit of P.aeruginosa AHIR partially superimposes with a three-domain monomer of spinach AHIR, a class II enzyme. This demonstrates that the so-called plant-specific insert in the middle of spinach AHIR is structurally and functionally equivalent to the C-terminal alpha-helical domain of P.aeruginosa AHIR, and the C-terminal alpha-helical domain was duplicated during evolution from the shorter, class I AHIRs to the longer, class II AHIRs. The dimeric unit of P.aeruginosa AHIR possesses a deep figure-of-eight knot, essentially identical with that in the spinach AHIR monomer. Thus, our work lowers the likelihood of the previous proposal that "domain duplication followed by exchange of a secondary structure element can be a source of such a knot in the protein structure" being correct.
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{{ABSTRACT_PUBMED_12691757}}
==About this Structure==
==About this Structure==
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[[Category: A deep figure-of-eight knot]]
[[Category: A deep figure-of-eight knot]]
[[Category: C-terminal alpha-helical domain]]
[[Category: C-terminal alpha-helical domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:41:21 2008''

Revision as of 08:41, 29 July 2008

Image:1np3.png

Template:STRUCTURE 1np3

Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa

Template:ABSTRACT PUBMED 12691757

About this Structure

1NP3 is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa., Ahn HJ, Eom SJ, Yoon HJ, Lee BI, Cho H, Suh SW, J Mol Biol. 2003 Apr 25;328(2):505-15. PMID:12691757

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