1nqw

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{{STRUCTURE_1nqw| PDB=1nqw | SCENE= }}
{{STRUCTURE_1nqw| PDB=1nqw | SCENE= }}
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'''Crystal Structure of Lumazine Synthase from Aquifex aeolicus in Complex with Inhibitor: 5-(6-D-ribitylamino-2,4(1H,3H)pyrimidinedione-5-yl)-1-pentyl-phosphonic acid'''
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===Crystal Structure of Lumazine Synthase from Aquifex aeolicus in Complex with Inhibitor: 5-(6-D-ribitylamino-2,4(1H,3H)pyrimidinedione-5-yl)-1-pentyl-phosphonic acid===
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==Overview==
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6,7-Dimethyl-8-ribityllumazine is the biosynthetic precursor of riboflavin, which, as a coenzyme, plays a vital role in the electron transfer process for energy production in all cellular organisms. The enzymes involved in lumazine biosynthesis have been studied in considerable detail. However, the conclusive mechanism of the reaction catalyzed by lumazine synthase has remained unclear. Here, we report four crystal structures of the enzyme from the hyperthermophilic bacterium Aquifex aeolicus in complex with different inhibitor compounds. The structures were refined at resolutions of 1.72 A, 1.85 A, 2.05 A and 2.2 A, respectively. The inhibitors have been designed in order to mimic the substrate, the putative reaction intermediates and the final product. Structural comparisons of the native enzyme and the inhibitor complexes as well as the kinetic data of single-site mutants of lumazine synthase from Bacillus subtilis showed that several highly conserved residues at the active site, namely Phe22, His88, Arg127, Lys135 and Glu138 are most likely involved in catalysis. A structural model of the catalytic process, which illustrates binding of substrates, enantiomer specificity, proton abstraction/donation, inorganic phosphate elimination, formation of the Schiff base and cyclization is proposed.
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{{ABSTRACT_PUBMED_12684006}}
==About this Structure==
==About this Structure==
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[[Category: Lumazine synthase]]
[[Category: Lumazine synthase]]
[[Category: Vitamin biosynthesis]]
[[Category: Vitamin biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:06:00 2008''

Revision as of 20:06, 28 July 2008

Image:1nqw.png

Template:STRUCTURE 1nqw

Crystal Structure of Lumazine Synthase from Aquifex aeolicus in Complex with Inhibitor: 5-(6-D-ribitylamino-2,4(1H,3H)pyrimidinedione-5-yl)-1-pentyl-phosphonic acid

Template:ABSTRACT PUBMED 12684006

About this Structure

1NQW is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

Reference

A structure-based model of the reaction catalyzed by lumazine synthase from Aquifex aeolicus., Zhang X, Meining W, Cushman M, Haase I, Fischer M, Bacher A, Ladenstein R, J Mol Biol. 2003 Apr 18;328(1):167-82. PMID:12684006

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