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1nrf

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[[Image:1nrf.gif|left|200px]]
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{{STRUCTURE_1nrf| PDB=1nrf | SCENE= }}
{{STRUCTURE_1nrf| PDB=1nrf | SCENE= }}
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'''C-terminal domain of the Bacillus licheniformis BlaR penicillin-receptor'''
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===C-terminal domain of the Bacillus licheniformis BlaR penicillin-receptor===
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==Overview==
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As in several staphylococci, the synthesis of the Bacillus licheniformis 749/I beta-lactamase is an inducible phenomenon regulated by a signal-transducing membrane protein BlaR. The C-terminal domain of this multimodular protein is an extracellular domain which specifically recognizes beta-lactam antibiotics. When it binds a beta-lactam, a signal is transmitted by the transmembrane region to the intracellular loops. In response, the hydrolytic activity of the BlaR large cytoplasmic L3 loop is induced, and a cascade of reactions is generated, leading to the transcription of the beta-lactamase gene. Here, we describe the crystal structure of the extracellular penicillin-receptor domain of BlaR (residues 346-601) at 2.5 A resolution in order to understand why this domain, whose folding is very similar to that of class D beta-lactamases, behaves as a highly sensitive penicillin-binding protein rather than a beta-lactamase. Two residues of the BlaR C-terminal domain, Thr452 and Thr542, modify the hydrophobic characteristic of the class D beta-lactamase active site. Both residues seem to be in part responsible for the lack of beta-lactamase activity of the BlaR protein due to the stability of the acyl-enzyme. Although further experimental data are needed to fully understand the transmembrane induction process, the comparison of the BlaR sensor domain structure with those of class D beta-lactamase complexes and penicillin-binding proteins provides interesting elements to hypothesize on possible signal transmission mechanisms.
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{{ABSTRACT_PUBMED_14596597}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of the sensor domain of the BlaR penicillin receptor from Bacillus licheniformis., Kerff F, Charlier P, Colombo ML, Sauvage E, Brans A, Frere JM, Joris B, Fonze E, Biochemistry. 2003 Nov 11;42(44):12835-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14596597 14596597]
Crystal structure of the sensor domain of the BlaR penicillin receptor from Bacillus licheniformis., Kerff F, Charlier P, Colombo ML, Sauvage E, Brans A, Frere JM, Joris B, Fonze E, Biochemistry. 2003 Nov 11;42(44):12835-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14596597 14596597]
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The penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in Bacillus licheniformis is embedded in the plasma membrane via a four-alpha-helix bundle., Hardt K, Joris B, Lepage S, Brasseur R, Lampen JO, Frere JM, Fink AL, Ghuysen JM, Mol Microbiol. 1997 Mar;23(5):935-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9076730 9076730]
[[Category: Bacillus licheniformis]]
[[Category: Bacillus licheniformis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Penicillin-binding protein]]
[[Category: Penicillin-binding protein]]
[[Category: Penicillin-receptor]]
[[Category: Penicillin-receptor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:53:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:35:09 2008''

Revision as of 23:35, 27 July 2008

Image:1nrf.png

Template:STRUCTURE 1nrf

C-terminal domain of the Bacillus licheniformis BlaR penicillin-receptor

Template:ABSTRACT PUBMED 14596597

About this Structure

1NRF is a Single protein structure of sequence from Bacillus licheniformis. Full crystallographic information is available from OCA.

Reference

Crystal structure of the sensor domain of the BlaR penicillin receptor from Bacillus licheniformis., Kerff F, Charlier P, Colombo ML, Sauvage E, Brans A, Frere JM, Joris B, Fonze E, Biochemistry. 2003 Nov 11;42(44):12835-43. PMID:14596597

The penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in Bacillus licheniformis is embedded in the plasma membrane via a four-alpha-helix bundle., Hardt K, Joris B, Lepage S, Brasseur R, Lampen JO, Frere JM, Fink AL, Ghuysen JM, Mol Microbiol. 1997 Mar;23(5):935-44. PMID:9076730

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