6de6
From Proteopedia
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<table><tr><td colspan='2'>[[6de6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_sp._4-9 Rhizobium sp. 4-9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DE6 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6de6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_sp._4-9 Rhizobium sp. 4-9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DE6 FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
| - | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4 | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6de6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6de6 OCA], [https://pdbe.org/6de6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6de6 RCSB], [https://www.ebi.ac.uk/pdbsum/6de6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6de6 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6de6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6de6 OCA], [https://pdbe.org/6de6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6de6 RCSB], [https://www.ebi.ac.uk/pdbsum/6de6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6de6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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Histamine dehydrogenase from the gram-negative bacterium Rhizobium sp. 4-9 (HaDHR) is a member of a small family of dehydrogenases containing a covalently attached FMN, and the only member so far identified to date that does not exhibit substrate inhibition. In this study, we present the 2.1 A resolution crystal structure of HaDHR. This new structure allowed for the identification of the internal electron transfer pathway to abiological ferrocene-based mediators. Alanine 437 was identified as the exit point of electrons from the Fe(4)S(4) cluster. The enzyme was modified with a Ser436Cys mutation to facilitate covalent attachment of a ferrocene moiety. When modified with Fc-maleimide, this new construct demonstrated direct electron transfer from the enzyme to a gold electrode in a histamine concentration-dependent manner without the need for any additional electron mediators. | Histamine dehydrogenase from the gram-negative bacterium Rhizobium sp. 4-9 (HaDHR) is a member of a small family of dehydrogenases containing a covalently attached FMN, and the only member so far identified to date that does not exhibit substrate inhibition. In this study, we present the 2.1 A resolution crystal structure of HaDHR. This new structure allowed for the identification of the internal electron transfer pathway to abiological ferrocene-based mediators. Alanine 437 was identified as the exit point of electrons from the Fe(4)S(4) cluster. The enzyme was modified with a Ser436Cys mutation to facilitate covalent attachment of a ferrocene moiety. When modified with Fc-maleimide, this new construct demonstrated direct electron transfer from the enzyme to a gold electrode in a histamine concentration-dependent manner without the need for any additional electron mediators. | ||
| - | Structure of Rhizobium sp. 4-9 histamine dehydrogenase and analysis of the electron transfer pathway to an abiological electron acceptor.,Goyal P, Deay D 3rd, Seibold S, Candido ACL, Lovell S, Battaile KP, Wilson GS, Richter ML, Petillo PA Arch Biochem Biophys. 2023 | + | Structure of Rhizobium sp. 4-9 histamine dehydrogenase and analysis of the electron transfer pathway to an abiological electron acceptor.,Goyal P, Deay D 3rd, Seibold S, Candido ACL, Lovell S, Battaile KP, Wilson GS, Richter ML, Petillo PA Arch Biochem Biophys. 2023 Jul 1;742:109612. doi: 10.1016/j.abb.2023.109612. Epub , 2023 May 3. PMID:37146865<ref>PMID:37146865</ref> |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
Current revision
2.1 A resolution structure of histamine dehydrogenase from Rhizobium sp. 4-9
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