1tcm
From Proteopedia
(New page: 200px<br /> <applet load="1tcm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tcm, resolution 2.2Å" /> '''CYCLODEXTRIN GLYCOSY...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1TCM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TCM OCA]]. | + | 1TCM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19]]. Structure known Active Sites: CA1, CA2, CA3, CA4, CT1, CT2, MB1, MB2, MB3, MB4, MB5 and MB6. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TCM OCA]]. |
==Reference== | ==Reference== | ||
The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251., Penninga D, van der Veen BA, Knegtel RM, van Hijum SA, Rozeboom HJ, Kalk KH, Dijkstra BW, Dijkhuizen L, J Biol Chem. 1996 Dec 20;271(51):32777-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8955113 8955113] | The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251., Penninga D, van der Veen BA, Knegtel RM, van Hijum SA, Rozeboom HJ, Kalk KH, Dijkstra BW, Dijkhuizen L, J Biol Chem. 1996 Dec 20;271(51):32777-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8955113 8955113] | ||
[[Category: Bacillus circulans]] | [[Category: Bacillus circulans]] | ||
| + | [[Category: Cyclomaltodextrin glucanotransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dijkstra, B.W.]] | [[Category: Dijkstra, B.W.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:47:13 2007'' |
Revision as of 09:42, 30 October 2007
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CYCLODEXTRIN GLYCOSYLTRANSFERASE W616A MUTANT FROM BACILLUS CIRCULANS STRAIN 251
Overview
The E-domain of cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19), from Bacillus circulans strain 251 is a putative raw starch binding, domain. Analysis of the maltose-dependent CGTase crystal structure, revealed that each enzyme molecule contained three maltose molecules, situated at contact points between protein molecules. Two of these, maltoses were bound to specific sites in the E-domain, the third maltose, was bound at the C-domain. To delineate the roles in raw starch binding, and cyclization reaction kinetics of the two maltose binding sites in the, E-domain, we replaced Trp-616 and Trp-662 of maltose binding site 1 and, Tyr-633 of maltose binding site 2 by alanines using site-directed, mutagenesis. Purified mutant CGTases were characterized with respect to, raw starch ... [(full description)]
About this Structure
1TCM is a [Single protein] structure of sequence from [Bacillus circulans] with CA as [ligand]. Active as [Cyclomaltodextrin glucanotransferase], with EC number [2.4.1.19]. Structure known Active Sites: CA1, CA2, CA3, CA4, CT1, CT2, MB1, MB2, MB3, MB4, MB5 and MB6. Full crystallographic information is available from [OCA].
Reference
The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251., Penninga D, van der Veen BA, Knegtel RM, van Hijum SA, Rozeboom HJ, Kalk KH, Dijkstra BW, Dijkhuizen L, J Biol Chem. 1996 Dec 20;271(51):32777-84. PMID:8955113
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