1nwd

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[[Image:1nwd.gif|left|200px]]
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{{STRUCTURE_1nwd| PDB=1nwd | SCENE= }}
{{STRUCTURE_1nwd| PDB=1nwd | SCENE= }}
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'''Solution Structure of Ca2+/Calmodulin bound to the C-terminal Domain of Petunia Glutamate Decarboxylase'''
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===Solution Structure of Ca2+/Calmodulin bound to the C-terminal Domain of Petunia Glutamate Decarboxylase===
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==Overview==
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Activation of glutamate decarboxylase (GAD) by calcium-bound calmodulin (CaM) is required for normal plant growth through regulation of gamma-aminobutyrate and glutamate metabolism. The interaction of CaM with the C-terminal domain of GAD is believed to induce dimerization of the enzyme, an event implicated for Ca(2+)-dependent enzyme activation. Here, we present the solution structure of CaM in complex with a dimer of peptides derived from the C-terminus of Petunia hybrida GAD. The 23 kDa ternary complex is pseudo-symmetrical with each domain of CaM bound to one of the two antiparallel GAD peptides, which form an X-shape with an interhelical angle of 60 degrees. To accommodate the dimeric helical GAD target, the two domains of CaM adopt an orientation markedly different from that seen in other CaM-target complexes. Although the dimeric GAD domain is much larger than previously studied CaM-binding peptides, the two CaM domains appear closer together and make a number of interdomain contacts not observed in earlier complexes. The present structure of a single CaM molecule interacting with two target peptides provides new evidence for the conformational flexibility of CaM as well as a structural basis for the ability of CaM to activate two enzyme molecules simultaneously.
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{{ABSTRACT_PUBMED_12684008}}
==About this Structure==
==About this Structure==
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1NWD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Petunia_x_hybrida Petunia x hybrida] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NWD OCA].
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1NWD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Petunia_x_hybrida Petunia x hybrida] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NWD OCA].
==Reference==
==Reference==
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[[Category: Glutamate decarboxylase]]
[[Category: Glutamate decarboxylase]]
[[Category: Nmr]]
[[Category: Nmr]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:03:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:37:06 2008''

Revision as of 10:37, 27 July 2008

Image:1nwd.png

Template:STRUCTURE 1nwd

Solution Structure of Ca2+/Calmodulin bound to the C-terminal Domain of Petunia Glutamate Decarboxylase

Template:ABSTRACT PUBMED 12684008

About this Structure

1NWD is a Protein complex structure of sequences from Petunia x hybrida and Xenopus laevis. Full experimental information is available from OCA.

Reference

Structural basis for simultaneous binding of two carboxy-terminal peptides of plant glutamate decarboxylase to calmodulin., Yap KL, Yuan T, Mal TK, Vogel HJ, Ikura M, J Mol Biol. 2003 Apr 18;328(1):193-204. PMID:12684008

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