1nx8

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[[Image:1nx8.jpg|left|200px]]
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{{Seed}}
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[[Image:1nx8.png|left|200px]]
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{{STRUCTURE_1nx8| PDB=1nx8 | SCENE= }}
{{STRUCTURE_1nx8| PDB=1nx8 | SCENE= }}
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'''Structure of carbapenem synthase (CarC) complexed with N-acetyl proline'''
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===Structure of carbapenem synthase (CarC) complexed with N-acetyl proline===
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==Overview==
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The proposed biosynthetic pathway to the carbapenem antibiotics proceeds via epimerization/desaturation of a carbapenam in an unusual process catalyzed by an iron- and 2-oxoglutarate-dependent oxygenase, CarC. Crystal structures of CarC complexed with Fe(II) and 2-oxoglutarate reveal it to be hexameric (space group C2221), consistent with solution studies. CarC monomers contain a double-stranded beta-helix core that supports ligands binding a single Fe(II) to which 2-oxoglutarate complexes in a bi-dentate manner. A structure was obtained with l-N-acetylproline acting as a substrate analogue. Quantum mechanical/molecular mechanical modeling studies with stereoisomers of carbapenams and carbapenems were used to investigate substrate binding. The combined work will stimulate further mechanistic studies and aid in the engineering of carbapenem biosynthesis.
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(as it appears on PubMed at http://www.pubmed.gov), where 12611886 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12611886}}
==About this Structure==
==About this Structure==
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[[Category: Wilmouth, R C.]]
[[Category: Wilmouth, R C.]]
[[Category: Jelly roll]]
[[Category: Jelly roll]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:05:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:00:54 2008''

Revision as of 21:00, 28 July 2008

Image:1nx8.png

Template:STRUCTURE 1nx8

Structure of carbapenem synthase (CarC) complexed with N-acetyl proline

Template:ABSTRACT PUBMED 12611886

About this Structure

1NX8 is a Single protein structure of sequence from Pectobacterium carotovorum. Full crystallographic information is available from OCA.

Reference

Crystal structure of carbapenem synthase (CarC)., Clifton IJ, Doan LX, Sleeman MC, Topf M, Suzuki H, Wilmouth RC, Schofield CJ, J Biol Chem. 2003 Jun 6;278(23):20843-50. Epub 2003 Feb 28. PMID:12611886

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